Explore
Validate
Learn
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunocytochemistry [2]
- Immunohistochemistry [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- GTX119118 - Provider product page
- Provider
- GeneTex
- Proper citation
- GeneTex Cat#GTX119118, RRID:AB_10617374
- Product name
- SEPT8 antibody
- Antibody type
- Polyclonal
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
No comments: Submit comment
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Sample (30 ug of whole cell lysate) A: HCT116 12% SDS PAGE GTX119118 diluted at 1:2000
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Various tissue extracts (10 ?g) were separated by 10% SDS-PAGE, and the membrane was blotted with SEPT8 antibody (GTX119118) diluted at 1:4000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Confocal immunofluorescence analysis (Olympus FV10i) of methanol-fixed HeLa, using 39332(GTX119118) antibody (Green) at 1:200 dilution. Alpha-tubulin filaments were labeled with GTX11304 (Red) at 1:2000.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- SEPT8 antibody detects SEPT8 protein by immunofluorescent analysis.Sample: DIV9 rat E18 primary cortical neurons were fixed in 4% paraformaldehyde at RT for 15 min.Green: SEPT8 protein stained by SEPT8 antibody (GTX119118) diluted at 1:500.Red: beta Tubulin 3/ Tuj1, stained by beta Tubulin 3/ Tuj1 antibody [GT886] (GTX631830) diluted at 1:500.Blue: Fluoroshield with DAPI (GTX30920).
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- SEPT8 antibody detects SEPT8 protein at cytosol on U87 xenograft by immunohistochemical analysis. Sample: Paraffin-embedded U87 xenograft. SEPT8 antibody (GTX119118) dilution: 1:500.
