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- Product number
- PA5-39247 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Anti-TOMM20 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µg
- Concentration
- 1 mg/mL
- Storage
- -20°C
Submitted references Identification of LACTB2, a metallo-β-lactamase protein, as a human mitochondrial endoribonuclease.
Levy S, Allerston CK, Liveanu V, Habib MR, Gileadi O, Schuster G
Nucleic acids research 2016 Feb 29;44(4):1813-32
Nucleic acids research 2016 Feb 29;44(4):1813-32
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of TOMM20 in extracts from HeLa cells, K562 cells and A549 cells using a TOMM20 polyclonal antibody (Product # PA5-39247).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 1. LACTB2 is a mitochondrial, soluble and monomeric protein. ( A )Mitochondria localization. HeLa cells were disrupted and the nuclear,cytoplasmic and mitochondria-containing fractions were separated and analyzedfor the localization of LACTB2, using an immunoblotting assay. Thenucleus-located protein, CPSF3L, was used as marker for nuclear proteins.Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) served as a cytosolic markerand cytochrome C (Cyt C) as a mitochondrial marker. ( B ) Immunoblotanalysis of mitochondrial extract following Proteinase K accessibility assay.TX-100, Triton X-100. PK, Proteinase K. PNPase is a mitochondrial proteinlocated mainly in the intermembrane space and a small amount in the matrix.HSP60 is a mitochondrial matrix protein. Tom20 is a mitochondrial outermembrane protein. ( C ) LACTB2 is a soluble protein. Immunoblottinganalysis of mitochondrial extract following alkaline sodium carbonate(Na 2 CO 3 ) extraction of soluble and peripheral membraneproteins. T, total extract. S, supernatant. P, pellet. Subunit 5 of the ATPsynthase (ATP5) was used as a marker for intrinsic membrane proteins and Cyt Cas a marker of soluble proteins. (D) Immunoblot analysis of the recombinantLACTB2 (Rec.) and the native protein in isolated mitochondria (Mit.) showingthe same size on SDS-PAGE. Therefore, the mitochondria targeting signal is notcleaved upon entering the mitochondria and remain an integral part of themature protein. ( E ) LACTB2 is present as a monomer
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 1. LACTB2 is a mitochondrial, soluble and monomeric protein. ( A ) Mitochondria localization. HeLa cells were disrupted and the nuclear, cytoplasmic and mitochondria-containing fractions were separated and analyzed for the localization of LACTB2, using an immunoblotting assay. The nucleus-located protein, CPSF3L, was used as marker for nuclear proteins. Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) served as a cytosolic marker and cytochrome C (Cyt C) as a mitochondrial marker. ( B ) Immunoblot analysis of mitochondrial extract following Proteinase K accessibility assay. TX-100, Triton X-100. PK, Proteinase K. PNPase is a mitochondrial protein located mainly in the intermembrane space and a small amount in the matrix. HSP60 is a mitochondrial matrix protein. Tom20 is a mitochondrial outer membrane protein. ( C ) LACTB2 is a soluble protein. Immunoblotting analysis of mitochondrial extract following alkaline sodium carbonate (Na 2 CO 3 ) extraction of soluble and peripheral membrane proteins. T, total extract. S, supernatant. P, pellet. Subunit 5 of the ATP synthase (ATP5) was used as a marker for intrinsic membrane proteins and Cyt C as a marker of soluble proteins. (D) Immunoblot analysis of the recombinant LACTB2 (Rec.) and the native protein in isolated mitochondria (Mit.) showing the same size on SDS-PAGE. Therefore, the mitochondria targeting signal is not cleaved upon entering the mitochondria and remain an integral part of the mature protein. ( E ) LACTB2 is
