M04039

antibody from Boster Biological Technology

Targeting: TOMM20 KIAA0016, MAS20, MOM19, TOM20

Western blot (Data presented on provider website)

Immunocytochemistry (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Data presented on provider website)

Antibody Data

Product number

M04039

Provider

Boster Biological Technology

Product name

Anti-TOMM20/Tom20 Rabbit Monoclonal Antibody

Antibody type

Monoclonal

Description

Monoclonal antibody for TOM20/TOMM20 detection. Host: Rabbit.Size: 100ug/vial. Tested applications: Flow Cytometry, IF, IHC, ICC, WB. Reactive species: Human, Mouse, Rat TOM20/TOMM20 information: Molecular Weight: 16298 MW; Subcellular Localization: Mitochondrion outer membrane ; Single-pass membrane protein .

Reactivity

Human, Mouse, Rat

Host

Rabbit

Antibody clone number

EOC-20

Vial size

100ug/vial

Concentration

0.5-1mg/ml, actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure.

Storage

At -20°C for one year. Avoid repeated freezing and thawing.

Immunohistochemistry

Supportive validation

Submitted by

Boster Biological Technology (provider)

Main image

Experimental details

Immunohistochemical analysis of paraffin-embedded human colon, using TOMM20 Antibody(M04039)TOMM20 was detected in paraffin-embedded tissue section. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-TOMM20 Antibody (M04039)overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.

Additional image