H00055055-M01

antibody from Abnova Corporation

Targeting: ZWILCH FLJ10036, KNTC1AP

Western blot (Supportive data in Antibodypedia)

ELISA (Supportive data in Antibodypedia)

Antibody Data

Product number

H00055055-M01

Provider

Abnova Corporation

Proper citation

Abnova Corporation Cat#H00055055-M01, RRID:AB_426001

Product name

ZWILCH monoclonal antibody (M01), clone 1C9

Antibody type

Monoclonal

Description

Mouse monoclonal antibody raised against a full length recombinant ZWILCH.

Antigen sequence

MAHNPNMTHLKINLPVTALPPLWVRCDSSDPEGTC
WLGAELITTNNSITGIVLYVVSCKADKNYSVNLEN
LKNLHKKRHHLSTVTSKGFAQYELFKSSALDDTIT
ASQTAIALDISWSPVDEILQIPPLSSTATLNIKVE
SGEPRGPLNHLYRELKFLLVLADGLRTGVTEWLEP
LEAKSAVELVQEFLNDLNKLDGFGDSTKKDTEVET
LKHDTAAVDRSVKRLFKVRSDLDFAEQLWCKMSSS
VISYQDLVKCFTLIIQSLQRGDIQPWLHSGSNSLL
SKLIHQSYHGTMDTVSLSGTIPVQMLLEIGLDKLK
KDYISFFIGQELASLNHLEYFIAPSVDIQEQVYRV
QKLHHILEILVSCMPFIKSQHELLFSLTQICIKYY
KQNPLDEQHIFQLPVRPTAVKNLYQSEKPQKWRVE
IYSGQKKIKTVWQLSDSSPIDHLNFHKPDFSELTL
NGSLEERIFFTNMVTCSQVHFK


Isotype

IgG

Antibody clone number

1C9

Storage

Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western Blot analysis of ZWILCH expression in transfected 293T cell line by ZWILCH monoclonal antibody (M01), clone 1C9.Lane 1: ZWILCH transfected lysate(54.224 KDa).Lane 2: Non-transfected lysate.

ELISA

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Detection limit for recombinant GST tagged ZWILCH is approximately 1ng/ml as a capture antibody.

Validation comment

Sandwich ELISA (Recombinant protein)

Protocol

Protocol