GTX118325

antibody from GeneTex

Targeting: HADH HADH1, HADHSC, SCHAD

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

GTX118325

Provider

GeneTex

Proper citation

GeneTex Cat#GTX118325, RRID:AB_10617989

Product name

HADH antibody

Antibody type

Polyclonal

Reactivity

Human, Mouse, Rat

Host

Rabbit

Western blot

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

HADH antibody detects HADH protein by western blot analysis.A. 30 ?g 293T whole cell lysate/extractB. 30 ?g A431 whole cell lysate/extract C. 30 ?g HeLa whole cell lysate/extractD. 30 ?g HepG2 whole cell lysate/extract10% SDS-PAGEHADH antibody (GTX118325) dilution: 1:1000 The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Sample (50 ?g of whole cell lysate) A: mouse liver 10% SDS PAGE GTX118325 diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Rat tissue extract (50 ?g) was separated by 12% SDS-PAGE, and the membrane was blotted with HADH antibody (GTX118325) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Rat tissue extract (50 ?g) was separated by 12% SDS-PAGE, and the membrane was blotted with HADH antibody (GTX118325) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Immunocytochemistry

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

HADH antibody detects HADH protein at Mitochondria by immunofluorescent analysis. Sample: HeLa cells were fixed in 2% paraformaldehyde/culture medium at 37¢J for 30 min.Green: HADH protein stained by HADH antibody (GTX118325) diluted at 1:500.Blue: Hoechst 33343 staining.

Immunohistochemistry

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

HADH antibody detects HADH protein at mitochondria on human colon carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded colon carcinoma. HADH antibody (GTX118325) dilution: 1:250.