H00003033-M01

antibody from Abnova Corporation

Targeting: HADH HADH1, HADHSC, SCHAD

Western blot (Supportive data in Antibodypedia)

ELISA (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

H00003033-M01

Provider

Abnova Corporation

Proper citation

Abnova Corporation Cat#H00003033-M01, RRID:AB_530074

Product name

HADHSC monoclonal antibody (M01), clone 4B5

Antibody type

Monoclonal

Description

Mouse monoclonal antibody raised against a partial recombinant HADHSC.

Antigen sequence

GKHPVSCKDTPGFIVNRLLVPYLMEAIRLYERGDA
SKEDIDTAMKLGAGYPMGPFELLDYVGLDTTKFIV
DGWHEMDAENPLHQPSPSLNKLVAENKFGKKTGEG
FYKYK


Isotype

IgG

Antibody clone number

4B5

Storage

Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.

References

Submitted references

Changes in peak fat oxidation in response to different doses of endurance training.

Rosenkilde M, Reichkendler MH, Auerbach P, Bonne TC, Sjödin A, Ploug T, Stallknecht BM
Scandinavian journal of medicine & science in sports 2015 Feb;25(1):41-52

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Independent effects of endurance training and weight loss on peak fat oxidation in moderately overweight men: a randomized controlled trial.

Nordby P, Rosenkilde M, Ploug T, Westh K, Feigh M, Nielsen NB, Helge JW, Stallknecht B
Journal of applied physiology (Bethesda, Md. : 1985) 2015 Apr 1;118(7):803-10

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BCAT1 promotes cell proliferation through amino acid catabolism in gliomas carrying wild-type IDH1.

Tönjes M, Barbus S, Park YJ, Wang W, Schlotter M, Lindroth AM, Pleier SV, Bai AHC, Karra D, Piro RM, Felsberg J, Addington A, Lemke D, Weibrecht I, Hovestadt V, Rolli CG, Campos B, Turcan S, Sturm D, Witt H, Chan TA, Herold-Mende C, Kemkemer R, König R, Schmidt K, Hull WE, Pfister SM, Jugold M, Hutson SM, Plass C, Okun JG, Reifenberger G, Lichter P, Radlwimmer B
Nature medicine 2013 Jul;19(7):901-908

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

HADHSC monoclonal antibody (M01), clone 4B5 Western Blot analysis of HADHSC expression in HepG2 ( Cat # L019V1 ).

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western Blot analysis of HADH expression in transfected 293T cell line by HADHSC monoclonal antibody (M01), clone 4B5.Lane 1: HADH transfected lysate(34.3 KDa).Lane 2: Non-transfected lysate.

ELISA

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Detection limit for recombinant GST tagged HADHSC is approximately 0.03ng/ml as a capture antibody.

Validation comment

Sandwich ELISA (Recombinant protein)

Protocol

Protocol

Immunocytochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunofluorescence of monoclonal antibody to HADHSC on HeLa cell. [antibody concentration 10 ug/ml]

Validation comment

Immunofluorescence

Protocol

Protocol

Immunoprecipitation

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunoprecipitation of HADH transfected lysate using anti-HADH monoclonal antibody and Protein A Magnetic Bead (U0007), and immunoblotted with HADH MaxPab rabbit polyclonal antibody.

Validation comment

Immunoprecipitation

Protocol

Protocol

Immunohistochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunoperoxidase of monoclonal antibody to HADHSC on formalin-fixed paraffin-embedded human colon. [antibody concentration 3 ug/ml]

Validation comment

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)

Protocol

Protocol