H00005607-M01

antibody from Abnova Corporation

Targeting: MAP2K5 HsT17454, MAPKK5, MEK5, PRKMK5

Western blot (Supportive data in Antibodypedia)

ELISA (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

H00005607-M01

Provider

Abnova Corporation

Proper citation

Abnova Corporation Cat#H00005607-M01, RRID:AB_425621

Product name

MAP2K5 monoclonal antibody (M01), clone M1-E6

Antibody type

Monoclonal

Description

Mouse monoclonal antibody raised against a full length recombinant MAP2K5.

Antigen sequence

MLWLALGPFPAMENQVLVIRIKIPNSGAVDWTVHS
GPQLLFRDVLDVIGQVLPEATTTAFEYEDEDGDRI
TVRSDEEMKAMLSYYYSTVMEQQVNGQLIEPLQIF
PRACKPPGERNIHGLKVNTRAGPSQHSSPAVSDSL
PSNSLKKSSAELKKILANGQMNEQDIRYRDTLGHG
NGGTVYKAYHVPSGKILAVKVILLDITLELQKQIM
SELEILYKCDSSYIIGFYGAFFVENRISICTEFMD
GGSLDVYRKMPEHVLGRIAVAVVKGLTYLWSLKIL
HRDVKPSNMLVNTRGQVKLCDFGVSTQLVNSIAKT
YVGTNAYMAPERISGEQYGIHSDVWSLGISFMELA
LGRFPYPQIQKNQGSLMPLQLLQCIVDEDSPVLPV
GESSEPFVHFITQCMRKQPKERPAPEELMGHPFIV
QFNDGNAAVVSMWVCRALEERRSQQGPP


Isotype

IgG

Antibody clone number

M1-E6

Storage

Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

MAP2K5 monoclonal antibody (M01), clone M1-E6 Western Blot analysis of MAP2K5 expression in Jurkat ( Cat # L017V1 ).

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

MAP2K5 monoclonal antibody (M01), clone M1-E6. Western Blot analysis of MAP2K5 expression in K-562 ( Cat # L009V1 ).

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western Blot analysis of MAP2K5 expression in transfected 293T cell line by MAP2K5 monoclonal antibody (M01), clone M1-E6.Lane 1: MAP2K5 transfected lysate (Predicted MW: 50.1 KDa).Lane 2: Non-transfected lysate.

ELISA

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Detection limit for recombinant GST tagged MAP2K5 is approximately 0.03ng/ml as a capture antibody.

Validation comment

Sandwich ELISA (Recombinant protein)

Protocol

Protocol

Immunocytochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunofluorescence of monoclonal antibody to MAP2K5 on HeLa cell . [antibody concentration 10 ug/ml]

Validation comment

Immunofluorescence

Protocol

Protocol