Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- ELISA [1]
- Immunocytochemistry [1]
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Validation data
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- Product number
- H00005607-M01 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#H00005607-M01, RRID:AB_540410
- Product name
- Mouse Monoclonal MEK5 Antibody
- Antibody type
- Monoclonal
- Description
- IgG purified. MAP2K5 - mitogen-activated protein kinase kinase 5
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.1 mg
- Storage
- Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: MEK5 Antibody (M1-E6) [H00005607-M01] - Analysis of MAP2K5 expression in transfected 293T cell line by MAP2K5 monoclonal antibody (M01), clone M1-E6.Lane 1: MAP2K5 transfected lysate (Predicted MW: 50.1 KDa).Lane 2: Non-transfected lysate.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: MEK5 Antibody (M1-E6) [H00005607-M01] - MAP2K5 monoclonal antibody (M01), clone M1-E6 Analysis of MAP2K5 expression in Jurkat.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Sandwich ELISA: MEK5 Antibody (M1-E6) [H00005607-M01] - Detection limit for recombinant GST tagged MAP2K5 is approximately 0.03ng/ml as a capture antibody.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunocytochemistry/Immunofluorescence: MEK5 Antibody (M1-E6) [H00005607-M01] - Analysis of monoclonal antibody to MAP2K5 on HeLa cell . Antibody concentration 10 ug/ml.