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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [8]
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- Product number
- PA5-62050 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- ZC3H14 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Immunogen sequence: RPSIEIYRPP ASRNADSGVH LNRLQFQQQQ NSIHAAKQLD MQSSWVYETG RLCEPEVLNS LEETYSPFFR NNSEKMSMED ENFRKRKLPV VSSVVKVK
- Concentration
- 0.1 mg/mL
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
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- Experimental details
- Western blot analysis of ZC3H14 in Lane 1: Marker (kDa) 250, 130, 95, 72, 55, 36, 28, 17, 10; Lane 2: Human cell line RT-4; Lane 3: Human cell line U-251MG sp; Lane 4: Human plasma (IgG/HSA depleted); Lane 5: Human liver tissue.; Lane 6: Human tonsil tissue. tissue. Samples were probed using a ZC3H14 Polyclonal Antibody (Product # PA5-62050).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent staining of ZC3H14 in human cell line MCF7 shows positivity in nucleus but excluded from the nucleoli. Samples were probed using a ZC3H14 Polyclonal Antibody (Product # PA5-62050).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of ZC3H14 in human liver using ZC3H14 Polyclonal Antibody (Product # PA5-62050).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of ZC3H14 in human colon using ZC3H14 Polyclonal Antibody (Product # PA5-62050).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of ZC3H14 in human testis and skeletal muscle tissues using ZC3H14 Polyclonal Antibody (Product # PA5-62050). Corresponding ZC3H14 RNA-seq data are presented for the same tissues.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of ZC3H14 in human colon, kidney, liver and testis using ZC3H14 Polyclonal Antibody (Product # PA5-62050) (A) shows similar protein distribution across tissues to an independent ZC3H14 Polyclonal Antibody (B).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of ZC3H14 in human prostate using ZC3H14 Polyclonal Antibody (Product # PA5-62050) shows weak to moderate nuclear positivity in glandular cells.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of ZC3H14 in human kidney using ZC3H14 Polyclonal Antibody (Product # PA5-62050) shows moderate nuclear positivity in cells in tubules.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of ZC3H14 in human testis using ZC3H14 Polyclonal Antibody (Product # PA5-62050) shows moderate to strong nuclear positivity in cells in seminiferous ducts.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of ZC3H14 in human skeletal muscle using ZC3H14 Polyclonal Antibody (Product # PA5-62050) shows no positivity in myocytes as expected.
