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LearnPA5-100708
antibody from Invitrogen Antibodies
Targeting: CCNL2
ania-6b, CCNM, CCNS, HLA-ISO, PCEE, SB138
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [1]
- Other assay [1]
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- Product number
- PA5-100708 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Cyclin L2 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- -20°C
Submitted references Exosome miR-23a-3p from Osteoblast Alleviates Spinal Cord Ischemia/Reperfusion Injury by Down-Regulating KLF3-Activated CCNL2 Transcription.
Wu C, Zhu Q, Yao Y, Shi Z, Jin C, Chen L
Developmental neuroscience 2022;44(3):121-130
Developmental neuroscience 2022;44(3):121-130
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of Cyclin L2 in NCI-H460 cell lysate (left lane: treated with the antigen-specific peptide). Samples were incubated with Cyclin L2 polyclonal antibody (Product # PA5-100708).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Fig. 4. KLF3 transcriptional activates CCNL2. a Combined with the results of HTFtarget database and GEO database, genes that were significantly upregulated in IRI and regulated by KLF3 transcription were screened. b The expression levels of S100A4, ID3, CCNL2, and TRAPPC3 in IRI were displayed by GEO database. c KLF3 expression was examined by q-PCR after ChIP assay in OGD/R-AGE1.HN cells. d The expression of CCNL2 in GE1.HN and OGD/R-AGE1.HN cells was detected by q-PCR. e , f In OGD/R-AGE1.HN cells, q-PCR and Western blot assay were conducted to detect the expression of CCNL2 before and after KLF3 interference. g Binding sites between KLF3 and CCNL2 promoter were predicted by JASPAR database. h In 293T cells transfected with pcDNA3.1-KLF3, dual-luciferase reporter assay was taken to detect the luciferase activity when co-transfected with pGL3, pGL3-CCNL2 promoter-WT or pGL3-CCNL2 promoter-MUT. i The expression of KLF3 was measured by Western blot assay after DNA pull-down in OGD/R-AGE1.HN cells. j CCNL2 expression under different conditions was detected by q-PCR. Two-way ANOVA ( c , h ); Student's t test ( d , e ); one-way ANOVA ( j ). ** p < 0.01. ANOVA, analysis of variance.
