PA5-112465

antibody from Invitrogen Antibodies

Targeting: MACROH2A1 H2AFY, macroH2A1.2

Western blot (Supportive data in Antibodypedia)

ELISA (Recommended by provider)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Data presented on provider website)

Immunohistochemistry (Recommended by provider)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-112465

Provider

Invitrogen Antibodies

Product name

Acetyl-Histone Macro-H2A.1 (Lys13) Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Synthetic peptide

Reactivity

Human

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

0.12 mg/mL

Storage

-20°C or -80°C if preferred

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot analysis of Acetyl Histone Macro-H2A.1 (Lys13) in multiple sample types using a Acetyl Histone Macro-H2A.1 (Lys13) polyclonal antibody (Product # PA5-112465) at a concentration of 1.25 µg/ml. A goat anti rabbit polyclonal secondary antibody was used at a dilution of 1:50,000. Sample types that were tested include: Hela whole cell lysate, 293 whole cell lysate, A549 whole cell lysate, Jurkat whole cell lysate, HepG2 whole cell lysate (all treated with 30mM sodium butyrate for 4h) .

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemichal analysis of Acetyl Histone Macro-H2A.1 (Lys13) in Hela cells using a Polyclonal antibody (Product # PA5-112465) at a dilution of 1:5. Hela cells (treated with 30mM sodium butyrate for 4 hour) performed on a Leica BondTM system. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemichal analysis of Acetyl Histone Macro-H2A.1 (Lys13) in Hela cells using a Polyclonal antibody (Product # PA5-112465) at a dilution of 1:2.5. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated Goat Anti-Rabbit IgG(H+L). Cells were also counter-stained with DAPI.

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunoprecipitation analysis of Acetyl Histone Macro-H2A.1 (Lys13) in 293 whole cell lysate using a Polyclonal antibody (Product #PA5-112465). An HRP-conjugated Protein G antibody was used as the secondary antibody (1:2,000). Lane 1: Rabbit control IgG, Lane 2: 5 µg primary antibody and 500 µg of 293 whole cell lysate, Lane 3:20 µg of 293 whole cell lysate.