PAB20539

antibody from Abnova Corporation

Targeting: SMIM19 C8orf40

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PAB20539

Provider

Abnova Corporation

Proper citation

Abnova Corporation Cat#PAB20539, RRID:AB_10965473

Product name

C8orf40 polyclonal antibody

Antibody type

Polyclonal

Description

Rabbit polyclonal antibody raised against recombinant C8orf40.

Antigen sequence

KRNKRRIMRIFSVPPTEETLSEPNFYDTISKIRLR
QQLEMYSISRKYDYQQPQNQADSVQLSLE


Isotype

IgG

Storage

Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western blot analysis of Lane 1: Negative control (vector only transfected HEK293T lysate), Lane 2: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells with C8orf40 polyclonal antibody (Cat # PAB20539).

Immunocytochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunofluorescent staining of human cell line A-431 with C8orf40 polyclonal antibody (Cat # PAB20539) at 1-4 ug/mL dilution shows positivity in nucleoli, cytoplasm.

Validation comment

Immunofluorescence

Immunohistochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunohistochemical staining of human rectum with C8orf40 polyclonal antibody (Cat # PAB20539) shows moderate cytoplasmic positivity in glandular cells at 1:50-1:200 dilution.

Validation comment

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)