Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [6]
- Immunocytochemistry [1]
- Immunohistochemistry [4]
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Validation data
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- Product number
- 25654-1-AP - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- UACA Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Other
- Description
- Immunogen sequence: LSQKYTEVK NVKEKLVEEN AKQTSEILAV QNLLQKQHVP LEQVEALKKS LNGTIENLKE ELKSMQRCYE KEQQTVTKLH QLLENQKNSS VPLAEHLQIK EAFEKEVGII KASLREKEEE SQNKMEEVSK LQSEVQNTKQ ALKKLETREV VDLSKYKATK SDLETQISSL NEKLANLNRK YEEVCEEVLH AKKKEISAKD EKELLHFSIE QEIKDQKERC DKSLTTITEL QRRIQESAKQ IEAKDNKITE LLNDVERLKQ ALNGLSQLTY TSGNPTKRQS QLIDTLQHQV KSLEQQLADA DRQHQEVIAI YRTHLLSAAQ GHMDEDVQEA LLQIIQMRQG LVC (1075-1416 aa encoded by BC113407)
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 150 µL
- Concentration
- 0.5 mg/mL
- Storage
- -20°C
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- HEK-293 cells were subjected to SDS PAGE followed by western blot with 25654-1-AP (UACA Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- HeLa cells were subjected to SDS PAGE followed by western blot with 25654-1-AP ( UACA Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Y79 cells were subjected to SDS PAGE followed by western blot with 25654-1-AP (UACA Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Neuro-2a cells were subjected to SDS PAGE followed by western blot with 25654-1-AP (UACA Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Y79 cells were subjected to SDS PAGE followed by western blot with 25654-1-AP (UACA Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- A375 cells were subjected to SDS PAGE followed by western blot with 25654-1-AP (UACA Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of A375 cells using 25654-1-AP (UACA antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human kidney tissue slide using 25654-1-AP (UACA antibody) at dilution of 1:200 (under 10x lens).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human kidney tissue slide using 25654-1-AP (UACA antibody) at dilution of 1:200 (under 40x lens).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human colon tissue slide using 25654-1-AP (UACA antibody) at dilution of 1:200 (under 10x lens).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human colon tissue slide using 25654-1-AP ( UACA antibody) at dilution of 1:200 (under 40x lens).