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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Immunohistochemistry [4]
- Flow cytometry [2]
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- Product number
- TA501951 - Provider product page
- Provider
- OriGene
- Proper citation
- OriGene Cat#TA501951, RRID:AB_11126829
- Product name
- SULT1A1 mouse monoclonal antibody, clone OTI9B7 (formerly 9B7)
- Antibody type
- Monoclonal
- Description
- SULT1A1 mouse monoclonal antibody, clone OTI9B7 (formerly 9B7)
- Host
- Mouse
- Conjugate
- Unconjugated
- Epitope
- SULT1A1
- Isotype
- IgG
- Antibody clone number
- OTI9B7
- Vial size
- 100 µl
- Concentration
- 0.49 mg/ml
Submitted references The Clock Protein Bmal1 Regulates Circadian Expression and Activity of Sulfotransferase 1a1 in Mice.
Guo L, Yu F, Zhang T, Wu B
Drug metabolism and disposition: the biological fate of chemicals 2018 Oct;46(10):1403-1410
Drug metabolism and disposition: the biological fate of chemicals 2018 Oct;46(10):1403-1410
No comments: Submit comment
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-SULT1A1 monoclonal antibody.
- Validation comment
- WB
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SULT1A1 (RC201601, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SULT1A1.
- Validation comment
- WB
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Anti-SULT1A1 mouse monoclonal antibody (TA501951) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY SULT1A1(RC201601 ).
- Validation comment
- IF
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-SULT1A1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA501951)
- Validation comment
- IHC
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-SULT1A1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA501951)
- Validation comment
- IHC
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded Human bladder tissue within the normal limits using anti-SULT1A1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA501951)
- Validation comment
- IHC
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded Human prostate tissue within the normal limits using anti-SULT1A1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA501951)
- Validation comment
- IHC
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Flow cytometric Analysis of Jurkat cells, using anti-SULT1A1 antibody(TA501951),(Red), compared to a nonspecific negative control antibody(TA50011),(Blue).
- Validation comment
- FC
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- HEK293T cells transfected with either RC201601 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-SULT1A1 antibody(TA501951), and then analyzed by flow cytometry.
- Validation comment
- FC
