10911-2-AP

antibody from Invitrogen Antibodies

Targeting: SULT1A1 P-PST, STP, STP1

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Data presented on provider website)

Immunohistochemistry (Recommended by provider)

Flow cytometry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

10911-2-AP

Provider

Invitrogen Antibodies

Product name

SULT1A1 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Other

Description

Immunogen sequence: MELIQDTSR PPLEYVKGVP LIKYFAEALG PLQSFQARPD DLLISTYPKS GTTWVSQILD MIYQGGDLEK CHRAPIFMRV PFLEFKAPGI PSGMETLKDT PAPRLLKTHL PLALLPQTLL DQKVKVVYVA RNAKDVAVSY YHFYHMAKVH PEPGTWDSFL EKFMVGEVSY GSWYQHVQEW WELSRTHPVL YLFYEDMKEN PKREIQKILE FVGHSLPEET VDFVVQHTSF KEMKKNPMTN YTTVPQEFMD HSISPFMRKG MAGDWKTTFT VAQNERFDAD YAEKMAGCSL SFRSEL (1-295 aa encoded by B C000923)

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

150 µL

Concentration

0.2 mg/mL

Storage

-20°C

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Mouse liver tissue were subjected to SDS PAGE followed by western blot with 10911-2-AP (SULT1A1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Rat liver tissue were subjected to SDS PAGE followed by western blot with 10911-2-AP (SULT1A1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

WB result of SULT1A1 antibody (10911-2-AP, 1:600) with si-control and si-SULT1A1 transfected Jurkat cells.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of HepG2 cells, using SULT1A1 antibody 10911-2-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IGG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

Flow cytometry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

1X10^6 HepG2 cells were stained with 0.2ug SULT1A1 antibody (10911-2-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L) with dilution 1:1000.

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

IP result of anti-SULT1A1 (IP:10911-2-AP, 3ug; Detection:10911-2-AP 1:1000) with mouse liver tissue lysate 12500ug.