Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [2]
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Validation data
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- Product number
- AF5546 - Provider product page
- Provider
- R&D Systems
- Product name
- Human Cytosolic Sulfotransferase 1A1/SULT1A1 Antibody
- Antibody type
- Polyclonal
- Description
- Antigen Affinity-purified. Detects human Cytosolic Sulfotransferase 1A1/SULT1A1 in direct ELISAs and Western blots. In direct ELISAs, less than 5% cross-reactivity with recombinant human SULT1E1 is observed.
- Reactivity
- Human
- Host
- Goat
- Conjugate
- Unconjugated
- Antigen sequence
P50225
- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references Contribution of nutritional deficit to the pathogenesis of the nonthyroidal illness syndrome in critical illness: a rabbit model study.
Mebis L, Eerdekens A, Güiza F, Princen L, Derde S, Vanwijngaerden YM, Vanhorebeek I, Darras VM, Van den Berghe G, Langouche L
Endocrinology 2012 Feb;153(2):973-84
Endocrinology 2012 Feb;153(2):973-84
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of Human Cytosolic Sulfotransferase 1A1/SULT1A1 by Western Blot. Western blot shows lysates of HEK293 human embryonic kidney cell line and human liver tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human Cytosolic Sulfotransferase 1A1/SULT1A1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5546) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for Cytosolic Sulfotransferase 1A1/SULT1A1 at approximately 37 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of Human Cytosolic Sulfotransferase 1A1/SULT1A1 by Simple WesternTM. Simple Western lane view shows lysates of human liver tissue, loaded at 0.2 mg/mL. A specific band was detected for Cytosolic Sulfotransferase 1A1/SULT1A1 at approximately 38 kDa (as indicated) using 10 µg/mL of Goat Anti-Human Cytosolic Sulfotransferase 1A1/SULT1A1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5546) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.