16386-1-AP

antibody from Invitrogen Antibodies

Targeting: RPL23A L23A

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

16386-1-AP

Provider

Invitrogen Antibodies

Product name

RPL23A Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Other

Description

Immunogen sequence: MAPKAKKEA PAPPKAEAKA KALKAKKAVL KGVHSHKKKK IRTSPTFRRP KTLRLRRQPK YPRKSAPRRN KLDHYAIIKF PLTTESAMKK IEDNNTLVFI VDVKANKHQI KQAVKKLYDI DVAKVNTLIR PDGEKKAYVR LAPDYDALDV ANKIGII (1-156 aa encoded by BC014459)

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

150 µL

Concentration

0.13 mg/mL

Storage

-20°C

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

HepG2 cells were subjected to SDS PAGE followed by western blot with 16386-1-AP (RPL23A antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Human liver tissue were subjected to SDS PAGE followed by western blot with 16386-1-AP (RPL23A antibody) at dilution of 1:0 incubated at room temperature for 1.5 hours.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of HepG2 cells using 16386-1-AP (RPL23A antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human liver using 16386-1-AP (RPL23A antibody) at dilution of 1:100 (under 10x lens).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human liver using 16386-1-AP (RPL23A antibody) at dilution of 1:100 (under 40x lens).

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

IP result of anti-RPL23A (IP:16386-1-AP, 4ug; Detection:16386-1-AP 1:500) with HepG2 cells lysate 3200ug.