Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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Validation data
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- Product number
- PAB29430 - Provider product page
- Provider
- Abnova Corporation
- Product name
- ANLN polyclonal antibody
- Antibody type
- Polyclonal
- Description
- Rabbit polyclonal antibody raised against recombinant human ANLN.
- Antigen sequence
DLFSDVLEEGELDMEKSQEEMDQALAESSEEQEDA
LNISSMSLLAPLAQTVGVVSPESLVSTPRLELKDT
SRSDESPKPGKFQRTRVPRAESGDSLGSEDRDLLY
SIDAYRSQRFKETERPSIKQVIVRKEDV- Isotype
- IgG
- Storage
- Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Western blot analysis of Lane 1: Human cell line RT-4 Lane 2: Human cell line U-251MG with ANLN polyclonal antibody (Cat # PAB29430) at 1:250-1:500 dilution.
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Immunofluorescent staining of human cell line U-2 OS with ANLN polyclonal antibody (Cat # PAB29430) at 1-4 ug/mL concentration shows positivity in nucleus but excluded from the nucleoli.
- Validation comment
- Immunofluorescence
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Immunohistochemical staining of human testis with ANLN polyclonal antibody (Cat # PAB29430) shows moderate nuclear positivity in cells in seminiferous ducts at 1:200-1:500 dilution.
- Validation comment
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)