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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [2]
- ELISA [1]
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- Product number
- H00065220-M01 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#H00065220-M01, RRID:AB_606635
- Product name
- NADK monoclonal antibody (M01), clone 5F4
- Antibody type
- Monoclonal
- Description
- Mouse monoclonal antibody raised against a full length recombinant NADK.
- Antigen sequence
MEMEQEKMTMNKELSPDAAAYCCSACHGDETWSYN
HPIRGRAKSRSLSASPALGSTKEFRRTRSLHGPCP
VTTFGPKACVLQNPQTIMHIQDPASQRLTWNKSPK
SVLVIKKMRDASLLQPFKELCTHLMEENMIVYVEK
KVLEDPAIASDESFGAVKKKFCTFREDYDDISNQI
DFIICLGGDGTLLYASSLFQGSVPPVMAFHLGSLG
FLTPFSFENFQSQVTQVIEGNAAVVLRSRLKVRVV
KELRGKKTAVHNGLGEKGSQAAGLDMDVGKQAMQY
QVLNEVVIDRGPSSYLSNVDVYLDGHLITTVQGDG
VIVSTPTGSTAYAAAAGASMIHPNVPAIMITPICP
HSLSFRPIVVPAGVELKIMLSPEARNTAWVSFDGR
KRQEIRHGDSISITTSCYPLPSICVRDPVSDWFES
LAQCLHWNVRKKQAHFEEEEEEEEEG- Isotype
- IgG
- Antibody clone number
- 5F4
- Storage
- Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
Submitted references NAD kinase levels control the NADPH concentration in human cells.
Pollak N, Niere M, Ziegler M
The Journal of biological chemistry 2007 Nov 16;282(46):33562-71
The Journal of biological chemistry 2007 Nov 16;282(46):33562-71
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- NADK monoclonal antibody (M01), clone 5F4. Western Blot analysis of NADK expression in Raw 264.7 ( Cat # L024V1 ).
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Western Blot analysis of NADK expression in transfected 293T cell line by NADK monoclonal antibody (M01), clone 5F4.Lane 1: NADK transfected lysate(49.2 KDa).Lane 2: Non-transfected lysate.
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Detection limit for recombinant GST tagged NADK is approximately 0.3ng/ml as a capture antibody.
- Validation comment
- Sandwich ELISA (Recombinant protein)
- Protocol
- Protocol
