TA502215

antibody from OriGene

Targeting: PACSIN3 SDPIII

Western blot (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

TA502215

Provider

OriGene

Proper citation

OriGene Cat#TA502215, RRID:AB_11128176

Product name

PACSIN3 mouse monoclonal antibody, clone OTI4A7 (formerly 4A7)

Antibody type

Monoclonal

Description

PACSIN3 mouse monoclonal antibody, clone OTI4A7 (formerly 4A7)

Reactivity

Canine

Host

Mouse

Conjugate

Unconjugated

Epitope

PACSIN3

Isotype

IgG

Antibody clone number

OTI4A7

Vial size

100 µl

Concentration

0.55 mg/ml

Western blot

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-PACSIN3 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).

Validation comment

WB

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PACSIN3 (RC204103, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PACSIN3.

Validation comment

WB

Flow cytometry

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

HEK293T cells transfected with either RC204103 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-PACSIN3 antibody(TA502215), and then analyzed by flow cytometry.

Validation comment

FC