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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
- Flow cytometry [1]
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- Product number
- AP52148PU-N - Provider product page
- Provider
- OriGene
- Product name
- IEX1 (IER3) (N-term) rabbit polyclonal antibody, Aff - Purified
- Antibody type
- Polyclonal
- Description
- IEX1 (IER3) (N-term) rabbit polyclonal antibody, Aff - Purified
- Host
- Rabbit
- Conjugate
- Unconjugated
- Epitope
- IER3
- Antibody clone number
- NULL
- Vial size
- 400 µl
- Concentration
- lot specific
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Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Western blot analysis of IER3 / IEX1?Antibody (N-term) ??in mouse heart tissue lysates (35ug/lane). This demonstrates the IER3 antibody detected the IER3 protein (arrow).
- Validation comment
- WB
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Western blot analysisof IER3 / IEX1?Antibody (N-term) ??in A549 cell line lysates (35ug/lane). This demonstrates the IER3 antibody detected the IER3 protein (arrow).
- Validation comment
- WB
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Confocal immunofluorescent analysis of IER3 / IEX1?Antibody (N-term) ??with A549 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
- Validation comment
- IF
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis in formalin fixed and paraffin embedded human liver tissue reacted with IER3 / IEX1?Antibody (N-term) ?, which was?peroxidase conjugated to the secondary antibody and?followed by?AB staining.
- Validation comment
- IHC
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Flow cytometric analysis of A549 cells using IER3 / IEX1?Antibody (N-term) ??(right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
- Validation comment
- FC
