Explore
Validate
Learn
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
- Flow cytometry [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- AP52148PU-N - Provider product page
- Provider
- Acris Antibodies GmbH
- Proper citation
- Acris Antibodies GmbH Cat#AP52148PU-N, RRID:AB_11147013
- Product name
- anti IER3 / IEX1 (N-term)
- Antibody type
- Polyclonal
- Antigen
- KLH conjugated synthetic peptide between 45-73 amino acids from the N-terminal region of human IER3 / IEX1
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Vial size
- 0.4 ml
- Concentration
- lot specific
No comments: Submit comment
Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Western blot analysisof IER3 / IEX1 Antibody (N-term) Cat.-No AP52148PU-N in A549 cell line lysates (35ug/lane). This demonstrates the IER3 antibody detected the IER3 protein (arrow).
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Western blot analysis of IER3 / IEX1 Antibody (N-term) Cat.-No AP52148PU-N in mouse heart tissue lysates (35ug/lane). This demonstrates the IER3 antibody detected the IER3 protein (arrow).
Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Confocal immunofluorescent analysis of IER3 / IEX1 Antibody (N-term) Cat.-No AP52148PU-N with A549 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis in formalin fixed and paraffin embedded human liver tissue reacted with IER3 / IEX1 Antibody (N-term) Cat.-No AP52148PU-N, which was peroxidase conjugated to the secondary antibody and followed by AB staining.
Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Flow cytometric analysis of A549 cells using IER3 / IEX1 Antibody (N-term) Cat.-No AP52148PU-N (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
