Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [6]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
- Other assay [1]
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Validation data
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- Product number
- 20164-1-AP - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- SMCR7L/MID51 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Other
- Description
- Immunogen sequence: FDTDTFCPP RPKPVARKGQ VDLKKSRLRM SLQEKLLTYY RNRAAIPAGE QARAKQAAVD ICAELRSFLR AKLPDMPLRD MYLSGSLYDD LQVVTADHIQ LIVPLVLEQN LWSCIPGEDT IMNVPGFFLV RRENPEYFPR GSSYWDRCVV GGYLSPKTVA DTFEKVVAGS INWPAIGSLL DYVIRPAPPP EALTLEVQYE RDKHLFIDFL PSVTLGDTVL VAKPHRLAQY DNLWRLSLRP AETARLRALD QADSGCRSLC LKILKAICKS TPALGHLTAS QLTNVILHLA QEEADWSPDM LADRFLQALR GLISYLEAGV LPSALNPKVN LFAELTPEEI DELGYTLYCS LSEPEVLLQT (105-463 aa encoded by BC002587)
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 150 µL
- Concentration
- 0.24 mg/mL
- Storage
- -20°C
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 20164-1-AP (SMCR7L/MID51 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Mouse testis tissue were subjected to SDS PAGE followed by western blot with 20164-1-AP (SMCR7L/MID51 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Rat testis tissue were subjected to SDS PAGE followed by western blot with 20164-1-AP (SMCR7L/MID51 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Mouse heart tissue were subjected to SDS PAGE followed by western blot with 20164-1-AP (SMCR7L/MID51 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Rat liver tissue were subjected to SDS PAGE followed by western blot with 20164-1-AP (SMCR7L/MID51 antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- HT-1080 cells were subjected to SDS PAGE followed by western blot with 20164-1-AP (SMCR7L/MID51 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of Hela cells, using SMCR7L antibody 20164-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IGG (red).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human testis slide using 20164-1-AP (SMCR7L/MID51 antibody at dilution of 1:50.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- IP result of anti-SMCR7L/MID51 (IP: 20164-1-AP, 3 µg; Detection: 20164-1-AP 1:1000) with RAW 264.7 cells lysate 2400 µg.