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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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- Product number
- PA5-28558 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- KRT71 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: HepG2.
- Concentration
- 0.91 mg/mL
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using KRT71 Polyclonal Antibody (Product # PA5-28558). Sample (30 µg of whole cell lysate). Lane A: HepG2 . 7.5% SDS PAGE. KRT71 Polyclonal Antibody (Product # PA5-28558) diluted at 1:5,000.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- KRT71 Polyclonal Antibody detects KRT71 protein at cytoplasm by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: KRT71 protein stained by KRT71 Polyclonal Antibody (Product # PA5-28558) diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar = 10 µm.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- KRT71 Polyclonal Antibody detects KRT71 protein at membrane on human colon carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded colon carcinoma. KRT71 Polyclonal Antibody (Product # PA5-28558) dilution: 1:500. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
