Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
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Validation data
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- Product number
- ACC-009-25UL - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- CaV3.3 (CACNA1I) Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Other
- Description
- Reconstitution: 1 X 25 µL double distilled water (DDW), depending on the sample size. The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20C. The reconstituted solution can be stored at 4C for up to 1 week. For longer periods, small aliquots should be stored at -20C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 25 µL
- Concentration
- 0.75 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysisof rat brain membranes: - 1. Anti-CaV3.3 (CACNA1I) Antibody (#ACC-009), (1:200).2. Anti-CaV3.3 (CACNA1I) Antibody , preincubated with Cav3.3/CACNA1I Blocking Peptide (#BLP-CC009).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Expression of CaV3.3 in mouse hippocampus. Immunohistochemical staining of perfusion-fixed frozen mouse brain sections with Anti-CaV3.3 (CACNA1I) Antibody (#ACC-009), (1:400), followed by donkey Anti-rabbit-Cy3. A. Staining in the hippocampal CA3 region, showed Cav3.3 immunoreactivity (red) appeared in soma of pyramidal neuron profiles (horizontal arrows) and in dendrites (vertical arrows) projecting into the stratum radiatum (SR). B. Pre-incubation of the Antibody with Cav3.3/CACNA1I Blocking Peptide (#BLP-CC009), suppressed staining. Cell nuclei are stained with DAPI (blue). P = pyramidal layer.