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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [3]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
- Flow cytometry [1]
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- Product number
- PAB1864 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#PAB1864, RRID:AB_1571955
- Product name
- CDH4 polyclonal antibody
- Antibody type
- Polyclonal
- Description
- Rabbit polyclonal antibody raised against synthetic peptide of CDH4.
- Storage
- Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.
Submitted references Frequent aberrant methylation of the CDH4 gene promoter in human colorectal and gastric cancer.
R-cadherin influences cell motility via Rho family GTPases.
Mutation analysis of cadherin-4 reveals amino acid residues of EC1 important for the structure and function.
Miotto E, Sabbioni S, Veronese A, Calin GA, Gullini S, Liboni A, Gramantieri L, Bolondi L, Ferrazzi E, Gafà R, Lanza G, Negrini M
Cancer research 2004 Nov 15;64(22):8156-9
Cancer research 2004 Nov 15;64(22):8156-9
R-cadherin influences cell motility via Rho family GTPases.
Johnson E, Theisen CS, Johnson KR, Wheelock MJ
The Journal of biological chemistry 2004 Jul 23;279(30):31041-9
The Journal of biological chemistry 2004 Jul 23;279(30):31041-9
Mutation analysis of cadherin-4 reveals amino acid residues of EC1 important for the structure and function.
Kitagawa M, Natori M, Murase S, Hirano S, Taketani S, Suzuki ST
Biochemical and biophysical research communications 2000 May 10;271(2):358-63
Biochemical and biophysical research communications 2000 May 10;271(2):358-63
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Western blot analysis of CDH4 polyclonal antibody (Cat # PAB1864) in HepG2 cell line lysates (35 ug/lane). CDH4 (arrow) was detected using the purified polyclonal antibody.
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Immunofluorescence analysis of CDH4 polyclonal antibody (Cat # PAB1864) in human brain tissue. 0.025 mg/mL primary antibody was followed by Alexa-Fluor-546-conjugated donkey anti-rabbit lgG (H+L). Alexa-Fluor-546 emits orange fluorescence. Blue counterstaining is DAPI.
- Validation comment
- Immunofluorescence
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Formalin-fixed and paraffin-embedded human hepatocellular carcinoma tissue reacted with CDH4 polyclonal antibody (Cat # PAB1864), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
- Validation comment
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Flow cytometric analysis of HepG2 cells using CDH4 polyclonal antibody (Cat # PAB1864)(bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
- Validation comment
- Flow Cytometry
