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Western blot
ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [3]
- Immunocytochemistry [1]
- Immunohistochemistry [2]
- Other assay [1]
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- Product number
- 11661-1-AP - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- RRM2 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Other
- Description
- Immunogen sequence: MLSLRVPLA PITDPQQLQL SPLKGLSLVD KENTPPALSG TRVLASKTAR RIFQEPTEPK TKAAAPGVED EPLLRENPRR FVIFPIEYHD IWQMYKKAEA SFWTAEEVDL SKDIQHWESL KPEERYFISH VLAFFAASDG IVNENLVERF SQEVQITEAR CFYGFQIAME NIHSEMYSLL IDTYIKDPKE REFLFNAIET MPCVKKKADW ALRWIGDKEA TYGERVVAFA AVEGIFFSGS FASIFWLKKR GLMPGLTFSN ELISRDEGLH CDFACLMFKH LVHKPSEERV REIIINAVRI EQEFLTEALP VKLIGMNCTL MKQYIEFVAD RLMLELGFSK VFRVENPFDF MENISLEGKT NFFEKRVGEY QRMGVMSSPT ENSFTLDADF (1-389 aa encoded by BC030154)
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 150 µL
- Concentration
- 0.25 mg/mL
- Storage
- -20°C
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- A431 cells were subjected to SDS PAGE followed by western blot with 11661-1-AP (RRM2 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- K-562 cells were subjected to SDS PAGE followed by western blot with 11661-1-AP (RRM2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Various lysates were subjected to SDS PAGE followed by western blot with 11661-1-AP (RRM2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of (-20°C Methanol) fixed HepG2 cells using RRM2 antibody (Product # 11661-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human tonsillitis tissue slide using 11661-1-AP (RRM2 Antibody) at dilution of 1:50 (under 10x lens).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human tonsillitis tissue slide using 11661-1-AP ( RRM2 Antibody) at dilution of 1:50 (under 40x lens).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- IP result of anti-RRM2 (IP:11661-1-AP, 4ug; Detection:11661-1-AP 1:500) with K-562 cells lysate 1200ug.
