AF3078

antibody from R&D Systems

Targeting: FABP2 I-FABP

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Data presented on provider website)

Antibody Data

Product number

AF3078

Provider

R&D Systems

Product name

Human FABP2/I-FABP Antibody

Antibody type

Polyclonal

Description

Antigen Affinity-purified. Detects human FABP2/I-FABP in direct ELISAs and Western blots. In direct ELISAs, approximately 50% cross-reactivity with recombinant rat FABP2 is observed.

Reactivity

Human

Host

Goat

Conjugate

Unconjugated

Antigen sequence

P12104


Isotype

IgG

Vial size

100 ug

Concentration

LYOPH

Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Western blot

Supportive validation

Submitted by

R&D Systems (provider)

Main image

Experimental details

Detection of Human FABP2/I-FABP by Western Blot. Western blot shows lysates of human small intestine tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human FABP2/I-FABP Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3078) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for FABP2/I-FABP at approximately 14 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Supportive validation

Submitted by

R&D Systems (provider)

Main image

Experimental details

Detection of Human and Mouse FABP2/I-FABP by Simple Western^TM. Simple Western lane view shows lysates of human small intestine tissue and mouse small intestine tissue, loaded at 0.2 mg/mL. A specific band was detected for FABP2/I-FABP at approximately 18 kDa (as indicated) using 20 µg/mL of Goat Anti-Human FABP2/I-FABP Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3078) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Immunocytochemistry

Supportive validation

Submitted by

R&D Systems (provider)

Main image

Experimental details

FABP2/I-FABP in HCT-116 Human Cell Line. FABP2/I-FABP was detected in immersion fixed HCT-116 human colorectal carcinoma cell line using Goat Anti-Human FABP2/I-FABP Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3078) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.