NB100-2021-0.1mg

antibody from Novus Biologicals

Targeting: PIPOX LPIPOX

Western blot (Supportive data in Antibodypedia)

ELISA (Data presented on provider website)

Immunoprecipitation (Data presented on provider website)

Antibody Data

Product number

NB100-2021-0.1mg

Provider

Novus Biologicals

Product name

Goat Polyclonal Pipecolic acid oxidase Antibody

Antibody type

Polyclonal

Description

Delipidation and Defibrination.

Reactivity

Bacteria

Host

Goat

Conjugate

Biotin

Isotype

IgG

Vial size

0.1 mg

Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Western blot

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: Pipecolic acid oxidase Antibody [HRP] [NB100-2021] - Detection of Sarcosine Oxidase. Samples of 1 and 0.25 ug of purified Sarcosine Oxidase per lane were run by SDS-PAGE under reducing and non-reducing conditions and reduced samples contained 4% BME and were boiled for 5 minutes. Protein was transferred to nitrocellulose and probed with Goat anti Sarcosine Oxidase. Primary antibody was detected with Dylight 649 conjugated Donkey anti Goat (605-743-125 1:10K 1.5 hr RT in and imaged on the BioRad VersaDoc imaging system.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: Pipecolic acid oxidase Antibody [HRP] [NB100-2021] - Lane 1: Sarcosine Oxidase (Microbial). Load: 50 ng per lane. Primary antibody: Goat anti-Sarcosine Oxidase (Microbial) Antibody Biotin Conjugated at 1:1,000 overnight at 4C. Secondary antibody: HRP streptavidin secondary antibody at 1:40,000 for 30 min at RT. Block: incubated with blocking buffer for 30 min at RT. Predicted/Observed size: 45 kDa, 45 kDa for Sarcosine Oxidase monomeric alpha subunit.