11376-1-AP

antibody from Invitrogen Antibodies

Targeting: SULT1A4

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

11376-1-AP

Provider

Invitrogen Antibodies

Product name

SULT1A4 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Other

Description

Immunogen sequence: MELIQDTSR PPLEYVKGVP LIKYFAEALG PLQSFQARPD DLLINTYPKS GTTWVSQILD MIYQGGDLEK CNRAPIYVRV PFLEVNDPGE PSGLETLKDT PPPRLIKSHL PLALLPQTLL DQKVKVVYVA RNPKDVAVSY YHFHRMEKAH PEPGTWDSFL EKFMAGEVSY GSWYQHVQEW WELSRTHPVL YLFYEDMKEN PKREIQKILE FVGRSLPEET MDFMVQHTSF KEMKKNPMTN YTTVPQELMD HSISPFMRKG MAGDWKTTFT VAQNERFDAD YAEKMAGCSL SFRSEL (1-295 aa encoded by B C014471)

Reactivity

Human, Mouse

Host

Rabbit

Isotype

IgG

Vial size

150 µL

Concentration

0.45 mg/mL

Storage

-20°C

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Human pancreas tissue were subjected to SDS PAGE followed by western blot with 11376-1-AP (SULT1A4 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

HepG2 cells were subjected to SDS PAGE followed by western blot with 11376-1-AP (SULT1A4 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Human colon tissue were subjected to SDS PAGE followed by western blot with 11376-1-AP (SULT1A4 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Mouse pancreas tissue were subjected to SDS PAGE followed by western blot with 11376-1-AP (SULT1A4 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

BxPC-3 cells were subjected to SDS PAGE followed by western blot with 11376-1-AP (SULT1A4 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of HepG2 cells using 11376-1-AP (SULT1A4 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of HepG2 cells, using SULT1A4 antibody 11376-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human breast cancer tissue slide using 11376-1-AP ( SULT1A4 Antibody) at dilution of 1:200 (under 10x lens). heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human breast cancer tissue slide using 11376-1-AP ( SULT1A4 Antibody) at dilution of 1:200 (under 40x lens). heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).