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Blocking/NeutralizingAntibody data
- Antibody Data
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- References [3]
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- Western blot [3]
- ELISA [1]
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- Product number
- H00083440-M01 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#H00083440-M01, RRID:AB_536628
- Product name
- Mouse Monoclonal ADPGK Antibody
- Antibody type
- Monoclonal
- Description
- IgG purified. ADPGK - ADP-dependent glucokinase
- Reactivity
- Human, Mouse, Rat
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.1 mg
- Storage
- Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Submitted references Zinc finger nuclease mediated knockout of ADP-dependent glucokinase in cancer cell lines: effects on cell survival and mitochondrial oxidative metabolism.
A human skeletal muscle interactome centered on proteins involved in muscular dystrophies: LGMD interactome.
Expression and role in glycolysis of human ADP-dependent glucokinase.
Richter S, Morrison S, Connor T, Su J, Print CG, Ronimus RS, McGee SL, Wilson WR
PloS one 2013;8(6):e65267
PloS one 2013;8(6):e65267
A human skeletal muscle interactome centered on proteins involved in muscular dystrophies: LGMD interactome.
Blandin G, Marchand S, Charton K, Danièle N, Gicquel E, Boucheteil JB, Bentaib A, Barrault L, Stockholm D, Bartoli M, Richard I
Skeletal muscle 2013 Feb 15;3(1):3
Skeletal muscle 2013 Feb 15;3(1):3
Expression and role in glycolysis of human ADP-dependent glucokinase.
Richter S, Richter JP, Mehta SY, Gribble AM, Sutherland-Smith AJ, Stowell KM, Print CG, Ronimus RS, Wilson WR
Molecular and cellular biochemistry 2012 May;364(1-2):131-45
Molecular and cellular biochemistry 2012 May;364(1-2):131-45
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: ADPGK Antibody (1E4) [H00083440-M01] - Analysis of ADPGK over-expressed 293 cell line, cotransfected with ADPGK Validated Chimera RNAi ( Cat # H00083440-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with ADPGK monoclonal antibody (M01), clone 1E4 (Cat # H00083440-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: ADPGK Antibody (1E4) [H00083440-M01] - Analysis of ADPGK expression in transfected 293T cell line by ADPGK monoclonal antibody (M01), clone 1E4.Lane 1: ADPGK transfected lysate(54.1 KDa).Lane 2: Non-transfected lysate.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: ADPGK Antibody (1E4) [H00083440-M01] - ADPGK monoclonal antibody (M01), clone 1E4 Analysis of ADPGK expression in HeLa.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Sandwich ELISA: ADPGK Antibody (1E4) [H00083440-M01] - Detection limit for recombinant GST tagged ADPGK is 0.3 ng/ml as a capture antibody.
