H00085403-M05

antibody from Abnova Corporation

Targeting: EAF1

Western blot (Supportive data in Antibodypedia)

ELISA (Supportive data in Antibodypedia)

Antibody Data

Product number

H00085403-M05

Provider

Abnova Corporation

Proper citation

Abnova Corporation Cat#H00085403-M05, RRID:AB_1674812

Product name

EAF1 monoclonal antibody (M05), clone 1G2

Antibody type

Monoclonal

Description

Mouse monoclonal antibody raised against a full-length recombinant EAF1.

Antigen sequence

MNGTANPLLDREEHCLRLGESFEKRPRASFHTIRY
DFKPASIDTSCEGELQVGKGDEVTITLPHIPGSTP
PMTVFKGNKRPYQKDCVLIINHDTGEFVLEKLSSS
IQVKKTRAEGSSKIQARMEQQPTRPPQTSQPPPPP
PPMPFRAPTKPPVGPKTSPLKDNPSPEPQLDDIKR
ELRAEVDIIEQMSSSSGSSSSDSESSSGSDDDSSS
SGGEDNGPASPPQPSHQQPYNSRPAVANGTSRPQG
SNQLMNALRNDLQLSESGSDSDD


Isotype

IgG

Antibody clone number

1G2

Storage

Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western Blot analysis of EAF1 expression in transfected 293T cell line by EAF1 monoclonal antibody (M05), clone 1G2.Lane 1: EAF1 transfected lysate (Predicted MW: 29 KDa).Lane 2: Non-transfected lysate.

ELISA

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Detection limit for recombinant GST tagged EAF1 is 3 ng/ml as a capture antibody.

Validation comment

Sandwich ELISA (Recombinant protein)

Protocol

Protocol