PA5-80838

antibody from Invitrogen Antibodies

Targeting: MZB1 HSPC190, MEDA-7, MGC29506, PACAP, pERp1

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-80838

Provider

Invitrogen Antibodies

Product name

MZB1 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant full-length protein

Description

This product is preservative free. It is recommended to add sodium azide to avoid contamination (final concentration 0.05%-0.1%).

Concentration

1 mg/mL

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of MZB1 in Lane A: Molt4 Whole Cell Lysate (30 µg), Lane B: Raji Whole Cell Lysate (30 µg) . Samples were probed using a MZB1 Polyclonal Antibody (Product # PA5-80838) at a 1:500 dilution, followed by a Goat Anti-Rabbit IgG (H+L), Dylight 800 Secondary Antibody at a 1:10000 dilution. Western blot was performed under reducing conditions. Predicted band size:21 kDa. Observed band size:18 kDa.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot using MZB1 Polyclonal Antibody (Product # PA5-80838) at 1:500 dilution. Lane A: Molt4 Whole Cell Lysate, Lane B: Raji Whole Cell Lysate. Lysates/proteins at 30 μg per lane. Secondary Goat Anti-Rabbit IgG H&L (DyLight™ 800) at 1:10,000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 21 kDa. Observed band size: 18 kDa. (We are unsure of the identity of these extra bands).

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of human MZB1 in human spleen with MZB1 Polyclonal Antibody (Product # PA5-80838, 1:10,000, formalin-fixed paraffin embedded sections).

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

MZB1 Immunoprecipitation using: Lane A: 0.5 mg Raji Whole Cell Lysate, Lane B: 0.5 mg Daudi Whole Cell Lysate 2 µL with MZB1 Polyclonal Antibody (Product # PA5-80838) and 15 µL of 50 % Protein G agarose. Primary antibody: MZB1 Polyclonal Antibody, at 1:200 dilution. Secondary antibody: Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5,000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 20 kDa. Observed band size: 20 kDa.