Antibody data
- Antibody Data
- Antigen structure
- References [2]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
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Validation data
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- Product number
- AF5360 - Provider product page
- Provider
- R&D Systems
- Product name
- Human/Mouse Neuroplastin 65 Antibody
- Antibody type
- Polyclonal
- Description
- Antigen Affinity-purified. Detects human and mouse Neuroplastin 65 in direct ELISAs and Western blots. In direct ELISAs, less than 5% cross-reactivity with recombinant human Neuroplastin 55 is observed.
- Reactivity
- Human, Mouse
- Host
- Goat
- Conjugate
- Unconjugated
- Antigen sequence
NP_036560
- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references Neuroplastin Isoform Np55 Is Expressed in the Stereocilia of Outer Hair Cells and Required for Normal Outer Hair Cell Function.
Neuroplastin-65 and a mimetic peptide derived from its homophilic binding site modulate neuritogenesis and neuronal plasticity.
Zeng WZ, Grillet N, Dewey JB, Trouillet A, Krey JF, Barr-Gillespie PG, Oghalai JS, Müller U
The Journal of neuroscience : the official journal of the Society for Neuroscience 2016 Aug 31;36(35):9201-16
The Journal of neuroscience : the official journal of the Society for Neuroscience 2016 Aug 31;36(35):9201-16
Neuroplastin-65 and a mimetic peptide derived from its homophilic binding site modulate neuritogenesis and neuronal plasticity.
Owczarek S, Soroka V, Kiryushko D, Larsen MH, Yuan Q, Sandi C, Berezin V, Bock E
Journal of neurochemistry 2011 Jun;117(6):984-94
Journal of neurochemistry 2011 Jun;117(6):984-94
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of Human/Mouse Neuroplastin 65 by Western Blot. Western blot shows lysates of human brain and mouse brain tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human/Mouse Neuroplastin 65 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5360) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for Neuroplastin 65 at approximately 55 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Neuroplastin 65 in Human Brain. Neuroplastin 65 was detected in immersion fixed paraffin-embedded sections of human brain (cerebellum) using 3 µg/mL Goat Anti-Human/Mouse Neuroplastin 65 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5360) overnight at 4 °C. Before incubation with the primary antibody tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained with the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Lower panel shows a lack of labeling if primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.