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LearnPA5-31614
antibody from Invitrogen Antibodies
Targeting: RPRD1B
C20orf77, CREPT, dJ1057B20.2, DKFZp434P0735, FLJ44520, K-H, Kub5-Hera, NET60
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [3]
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- Product number
- PA5-31614 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- RPRD1B Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: 293T, HeLa, HepG2.
- Concentration
- 1 mg/mL
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using RPRD1B Polyclonal Antibody (Product # PA5-31614). Sample (30 µg of whole cell lysate). Lane A: 293T. Lane B: HeLa. Lane C: HepG2. 10% SDS PAGE. RPRD1B Polyclonal Antibody (Product # PA5-31614) diluted at 1:1,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- RPRD1B Polyclonal Antibody detects RPRD1B protein at nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: RPRD1B protein stained by RPRD1B Polyclonal Antibody (Product # PA5-31614) diluted at 1:500. Blue: Hoechst 33342 staining.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry (Paraffin) analysis of RPRD1B was performed in paraffin-embedded mouse cervix tissue using RPRD1B Polyclonal Antibody (Product # PA5-31614) at a dilution of 1:500.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded C2C12 xenograft, using RPRD1B (Product # PA5-31614) antibody at 1:500 dilution. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded SkHep1 xenograft, using RPRD1B (Product # PA5-31614) antibody at 1:500 dilution. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
