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Western blot
Chromatin ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [2]
- Immunoprecipitation [1]
- Immunohistochemistry [1]
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- Product number
- NB100-86984 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NB100-86984, RRID:AB_2266238
- Product name
- Rabbit Polyclonal MAZ Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 ul
- Concentration
- 1.0 mg/ml
- Storage
- Store at 4C. Do not freeze.
Submitted references The histone code reader SPIN1 controls RET signaling in liposarcoma.
Franz H, Greschik H, Willmann D, Ozretić L, Jilg CA, Wardelmann E, Jung M, Buettner R, Schüle R
Oncotarget 2015 Mar 10;6(7):4773-89
Oncotarget 2015 Mar 10;6(7):4773-89
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: MAZ Antibody [NB100-86984] - Detection of Human MAZ/SAF-1 on HeLa whole cell lysate using NB100-86984. MAZ/SAF-1 was also immunoprecipitated by another rabbit anti-MAZ/SAF-1 antibody.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: MAZ Antibody [NB100-86984] - Whole cell lysate (50 ug) from HeLa, 293T, and mouse NIH3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-MAZ antibody used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunoprecipitation: MAZ Antibody [NB100-86984] - Detection of human MAZ by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-MAZ antibody NB100-86984 (lot NB100-86984-2) used for IP at 6 ug per reaction. MAZ was also immunoprecipitated by a previous lot of this antibody (lot NB100-86984-1) and another rabbit anti-MAZ antibody. For blotting immunoprecipitated MAZ, NB100-86984 was used at 1 ug/ml. Detection: . Chemiluminescence with an exposure time of 3 minutes.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: MAZ Antibody [NB100-86984] - FFPE section of human prostate carcinoma. Antibody: Affinity purified rabbit antiMAZ/SAF-1 used at a dilution of 1:1,000 (1ug/ml). Detection: DAB
