H00000686-M01

antibody from Abnova Corporation

Targeting: BTD

Western blot (Supportive data in Antibodypedia)

Immunoprecipitation (Supportive data in Antibodypedia)

Antibody Data

Product number

H00000686-M01

Provider

Abnova Corporation

Proper citation

Abnova Corporation Cat#H00000686-M01, RRID:AB_425333

Product name

BTD monoclonal antibody (M01), clone 3B10-2B3

Antibody type

Monoclonal

Description

Mouse monoclonal antibody raised against a full length recombinant BTD.

Antigen sequence

MAHAHIQGGRRAKSRFVVCIMSGARSKLALFLCGC
YVVALGAHTGEESVADHHEAEYYVAAVYEHPSILS
LNPLALISRQEALELMNQNLDIYEQQVMTAAQKDV
QIIVFPEDGIHGFNFTRTSIYPFLDFMPSPQVVRW
NPCLEPHRFNDTEVLQRLSCMAIRGDMFLVANLGT
KEPCHSSDPRCPKDGRYQFNTNVVFSNNGTLVDRY
RKHNLYFEAAFDVPLKVDLITFDTPFAGRFGIFTC
FDILFFDPAIRVLRDYKVKHVVYPTAWMNQLPLLA
AIEIQKAFAVAFGINVLAANVHHPVLGMTGSGIHT
PLESFWYHDMENPKSHLIIAQVAKNPVGLIGAENA
TGETDPSHSKFLKILSGDPYCEKDAQEVHCDEATK
WNVNAPPTFHSEMMYDNFTLVPVWGKEGYLHVCSN
GLCCYLLYERPTLSKELYALGVFDGLHTVHGTYYI
QVCALVRCGGLGFDTCGQEITEATGIFEFHLWGNF
STSYIFPLFLTSGMTLEVPDQLGWENDHYFLRKSR
LSSGLVTAALYGRLYERD


Isotype

IgG

Antibody clone number

3B10-2B3

Storage

Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western Blot analysis of BTD expression in transfected 293T cell line by BTD monoclonal antibody (M01), clone 3B10-2B3.Lane 1: BTD transfected lysate(61.1 KDa).Lane 2: Non-transfected lysate.

Immunoprecipitation

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunoprecipitation of BTD transfected lysate using anti-BTD monoclonal antibody and Protein A Magnetic Bead (U0007), and immunoblotted with BTD MaxPab rabbit polyclonal antibody.

Validation comment

Immunoprecipitation

Protocol

Protocol