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- Antibody Data
- Antigen structure
- References [12]
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- Validations
- Immunohistochemistry [1]
- Flow cytometry [1]
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- Product number
- AF1245 - Provider product page
- Provider
- R&D Systems
- Product name
- Mouse Galectin-1 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. Detects mouse Galectin-1 in ELISAs and Western blots. In sandwich ELISAs, less than 5% cross-reactivity with recombinant human (rh) Galectin-1 and less than 0.5% cross-reactivity with recombinant mouse (rm) Galectin-3, rhGalectin-4, rmGalectin-7, and rhGalectin-8 is observed.
- Reactivity
- Mouse
- Host
- Goat
- Conjugate
- Unconjugated
- Antigen sequence
P16045- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references Galectin-1 Restricts Vascular Smooth Muscle Cell Motility Via Modulating Adhesion Force and Focal Adhesion Dynamics.
Silencing of galectin-1 inhibits retinal neovascularization and ameliorates retinal hypoxia in a murine model of oxygen-induced ischemic retinopathy.
Natural killer cells eradicate galectin-1-deficient glioma in the absence of adaptive immunity.
Mechanism of Ca²⁺-triggered ESCRT assembly and regulation of cell membrane repair.
Identification of galectin-1 as a critical factor in function of mouse mesenchymal stromal cell-mediated tumor promotion.
Impaired spatial and contextual memory formation in galectin-1 deficient mice.
Endogenous galectin-1 and acute inflammation: emerging notion of a galectin-9 pro-resolving effect.
Galectin-1 attenuates astrogliosis-associated injuries and improves recovery of rats following focal cerebral ischemia.
Expression of galectin-1, a new component of slit diaphragm, is altered in minimal change nephrotic syndrome.
Identification of a lectin causing the degeneration of neuronal processes using engineered embryonic stem cells.
Altered expression of galectin-3 induces cortical thymocyte depletion and premature exit of immature thymocytes during Trypanosoma cruzi infection.
Galectin-1 supports survival of naive T cells without promoting cell proliferation.
Tsai MS, Chiang MT, Tsai DL, Yang CW, Hou HS, Li YR, Chang PC, Lin HH, Chen HY, Hwang IS, Wei PK, Hsu CP, Lin KI, Liu FT, Chau LY
Scientific reports 2018 Jul 31;8(1):11497
Scientific reports 2018 Jul 31;8(1):11497
Silencing of galectin-1 inhibits retinal neovascularization and ameliorates retinal hypoxia in a murine model of oxygen-induced ischemic retinopathy.
Yang N, Zhang W, He T, Xing Y
Experimental eye research 2017 Jun;159:1-15
Experimental eye research 2017 Jun;159:1-15
Natural killer cells eradicate galectin-1-deficient glioma in the absence of adaptive immunity.
Baker GJ, Chockley P, Yadav VN, Doherty R, Ritt M, Sivaramakrishnan S, Castro MG, Lowenstein PR
Cancer research 2014 Sep 15;74(18):5079-90
Cancer research 2014 Sep 15;74(18):5079-90
Mechanism of Ca²⁺-triggered ESCRT assembly and regulation of cell membrane repair.
Scheffer LL, Sreetama SC, Sharma N, Medikayala S, Brown KJ, Defour A, Jaiswal JK
Nature communications 2014 Dec 23;5:5646
Nature communications 2014 Dec 23;5:5646
Identification of galectin-1 as a critical factor in function of mouse mesenchymal stromal cell-mediated tumor promotion.
Szebeni GJ, Kriston-Pál É, Blazsó P, Katona RL, Novák J, Szabó E, Czibula Á, Fajka-Boja R, Hegyi B, Uher F, Krenács L, Joó G, Monostori É
PloS one 2012;7(7):e41372
PloS one 2012;7(7):e41372
Impaired spatial and contextual memory formation in galectin-1 deficient mice.
Sakaguchi M, Arruda-Carvalho M, Kang NH, Imaizumi Y, Poirier F, Okano H, Frankland PW
Molecular brain 2011 Sep 1;4:33
Molecular brain 2011 Sep 1;4:33
Endogenous galectin-1 and acute inflammation: emerging notion of a galectin-9 pro-resolving effect.
Iqbal AJ, Sampaio AL, Maione F, Greco KV, Niki T, Hirashima M, Perretti M, Cooper D
The American journal of pathology 2011 Mar;178(3):1201-9
The American journal of pathology 2011 Mar;178(3):1201-9
Galectin-1 attenuates astrogliosis-associated injuries and improves recovery of rats following focal cerebral ischemia.
Qu WS, Wang YH, Ma JF, Tian DS, Zhang Q, Pan DJ, Yu ZY, Xie MJ, Wang JP, Wang W
Journal of neurochemistry 2011 Jan;116(2):217-26
Journal of neurochemistry 2011 Jan;116(2):217-26
Expression of galectin-1, a new component of slit diaphragm, is altered in minimal change nephrotic syndrome.
Shimizu M, Khoshnoodi J, Akimoto Y, Kawakami H, Hirano H, Higashihara E, Hosoyamada M, Sekine Y, Kurayama R, Kurayama H, Joh K, Hirabayashi J, Kasai K, Tryggvason K, Ito N, Yan K
Laboratory investigation; a journal of technical methods and pathology 2009 Feb;89(2):178-95
Laboratory investigation; a journal of technical methods and pathology 2009 Feb;89(2):178-95
Identification of a lectin causing the degeneration of neuronal processes using engineered embryonic stem cells.
Plachta N, Annaheim C, Bissière S, Lin S, Rüegg M, Hoving S, Müller D, Poirier F, Bibel M, Barde YA
Nature neuroscience 2007 Jun;10(6):712-9
Nature neuroscience 2007 Jun;10(6):712-9
Altered expression of galectin-3 induces cortical thymocyte depletion and premature exit of immature thymocytes during Trypanosoma cruzi infection.
Silva-Monteiro E, Reis Lorenzato L, Kenji Nihei O, Junqueira M, Rabinovich GA, Hsu DK, Liu FT, Savino W, Chammas R, Villa-Verde DM
The American journal of pathology 2007 Feb;170(2):546-56
The American journal of pathology 2007 Feb;170(2):546-56
Galectin-1 supports survival of naive T cells without promoting cell proliferation.
Endharti AT, Zhou YW, Nakashima I, Suzuki H
European journal of immunology 2005 Jan;35(1):86-97
European journal of immunology 2005 Jan;35(1):86-97
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Galectin-1 in Mouse Thymus. Galectin-1 was detected in perfusion fixed frozen sections of mouse thymus using Goat Anti-Mouse Galectin-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1245) at 5 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific labeling was localized to the cytoplasm of lymphocytes. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of Galectin-1 in Mouse Splenocytes by Flow Cytometry. Mouse splenocytes were stained with Rat Anti-Mouse CD3 APC-conjugated Monoclonal Antibody (Catalog # FAB4841A) and either (A) Normal Goat IgG Control (Catalog # AB-108-C) or (B) Goat Anti-Mouse Galectin-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1245) followed by Phycoerythrin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0107). To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with saponin.
