AF1152

antibody from R&D Systems

Targeting: LGALS1 GBP

Provider product page for AF1152

Western blot

Immunohistochemistry

Flow cytometry

Antibody data

Antibody Data
Antigen structure
References [9]
Comments [0]
Validations
Western blot [2]
Immunohistochemistry [2]
Flow cytometry [1]

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Product number: AF1152 - Provider product page
Provider: R&D Systems
Product name: Human Galectin-1 Antibody
Antibody type: Polyclonal
Description: Antigen Affinity-purified. Detects human Galectin-1 in direct ELISAs and Western blots. In direct ELISAs, approximately 40% cross-reactivity with recombinant mouse (rm) Galectin-1 is observed and less than 5% cross-reactivity with recombinant human (rh) Galectin-2, rhGalectin-3, rhGalectin-4, rhGalectin-7, rhGalectin-8, and rmGalectin-9 is observed.
Reactivity: Human
Host: Goat
Conjugate: Unconjugated
Antigen sequence: P09382
Isotype: IgG
Vial size: 100 ug
Concentration: LYOPH
Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.

LGALS1 protein structure - AF1152 shown in red.

Submitted references Treatment of B-cell precursor acute lymphoblastic leukemia with the Galectin-1 inhibitor PTX008.

Paz H, Joo EJ, Chou CH, Fei F, Mayo KH, Abdel-Azim H, Ghazarian H, Groffen J, Heisterkamp N
Journal of experimental & clinical cancer research : CR 2018 Mar 27;37(1):67

Serum galectin-1 in patients with multiple myeloma: associations with survival, angiogenesis, and biomarkers of macrophage activation.

Andersen MN, Ludvigsen M, Abildgaard N, Petruskevicius I, Hjortebjerg R, Bjerre M, Honoré B, Møller HJ, Andersen NF
OncoTargets and therapy 2017;10:1977-1982

Galectins-1, -3, and -7 Are Prognostic Markers for Survival of Ovarian Cancer Patients.

Schulz H, Schmoeckel E, Kuhn C, Hofmann S, Mayr D, Mahner S, Jeschke U
International journal of molecular sciences 2017 Jun 8;18(6)

Prototype and Chimera-Type Galectins in Placentas with Spontaneous and Recurrent Miscarriages.

Unverdorben L, Haufe T, Santoso L, Hofmann S, Jeschke U, Hutter S
International journal of molecular sciences 2016 Apr 28;17(5)

Graves' disease is associated with a defective expression of the immune regulatory molecule galectin-9 in antigen-presenting dendritic cells.

Leskela S, Serrano A, de la Fuente H, Rodríguez-Muñoz A, Ramos-Levi A, Sampedro-Nuñez M, Sánchez-Madrid F, González-Amaro R, Marazuela M
PloS one 2015;10(4):e0123938

Melanoma Cell Galectin-1 Ligands Functionally Correlate with Malignant Potential.

Yazawa EM, Geddes-Sweeney JE, Cedeno-Laurent F, Walley KC, Barthel SR, Opperman MJ, Liang J, Lin JY, Schatton T, Laga AC, Mihm MC, Qureshi AA, Widlund HR, Murphy GF, Dimitroff CJ
The Journal of investigative dermatology 2015 Jul;135(7):1849-1862

Tonsil-derived mesenchymal stem cells alleviate concanavalin A-induced acute liver injury.

Ryu KH, Kim SY, Kim YR, Woo SY, Sung SH, Kim HS, Jung SC, Jo I, Park JW
Experimental cell research 2014 Aug 1;326(1):143-54

Galectin-1-mediated cell death is increased by CD30-induced signaling in anaplastic large cell lymphoma cells but not in Hodgkin lymphoma cells.

Suzuki O, Hirsch B, Abe M, Dürkop H, Stein H
Laboratory investigation; a journal of technical methods and pathology 2012 Feb;92(2):191-9

Sialylation of beta1 integrins blocks cell adhesion to galectin-3 and protects cells against galectin-3-induced apoptosis.

Zhuo Y, Chammas R, Bellis SL
The Journal of biological chemistry 2008 Aug 8;283(32):22177-85

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Supportive validation

Submitted by: R&D Systems (provider)
Main image
Experimental details: Detection of Human Galectin-1 by Western Blot. Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line and A549 human lung carcinoma cell line. PVDF membrane was probed with 0.1 µg/mL of Goat Anti-Human Galectin-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1152) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for Galectin-1 at approximately 14 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Supportive validation

Submitted by: R&D Systems (provider)
Main image
Experimental details: Detection of Human Galectin-1 by Simple Western^TM. Simple Western lane view shows lysates of HeLa human cervical epithelial carcinoma cell line and A549 human lung carcinoma cell line, loaded at 0.2 mg/mL. A specific band was detected for Galectin-1 at approximately 17 kDa (as indicated) using 1 µg/mL of Goat Anti-Human Galectin-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1152) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Supportive validation

Submitted by: R&D Systems (provider)
Main image
Experimental details: Galectin-1 in Human Prostate Cancer Tissue. Galectin-1 was detected in immersion fixed paraffin-embedded sections of human prostate cancer tissue using Goat Anti-Human Galectin-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1152) at 5 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific labeling was localized to the cytoplasm of stromal cells and nuclei of epithelial cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Supportive validation

Submitted by: R&D Systems (provider)
Main image
Experimental details: Galectin-1 in Human Prostate Cancer Tissue. Galectin-1 was detected in immersion fixed paraffin-embedded sections of human prostate cancer tissue using 1.7 µg/mL Goat Anti-Human Galectin-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1152) overnight at 4 °C. Tissue was stained with the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Supportive validation

Submitted by: R&D Systems (provider)
Main image
Experimental details: Detection of Galectin-1 in Human Blood Monocytes by Flow Cytometry. Human peripheral blood monocytes were stained with Mouse Anti-Human CD14 PE-conjugated Monoclonal Antibody (Catalog # FAB3832P) and either (A) Normal Goat IgG Control (Catalog # AB-108-C) or (B) Goat Anti-Human Galectin-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1152) followed by Allophycocyanin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0108). To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with saponin.