V2569

antibody from NSJ Bioreagents

Targeting: HLA-B AS

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Recommended by provider)

Flow cytometry (Recommended by provider)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

V2569

Provider

NSJ Bioreagents

Product name

HLA-ABC Antibody (MHC I)

Antibody type

Monoclonal

Antigen

Human peripheral blood leukocytes were used as the immunogen for the HLA-ABC antibody.

Description

Protein G purified antibody

Reactivity

Human, Mouse

Host

Mouse

Conjugate

Unconjugated

Antibody clone number

246-B8.E7

Vial size

20 µg, 100 µg (with sodium azide), 100 µg (without sodium azide), 7 ml (prediluted for IHC use, if applicable)

Storage

Store the HLA-ABC antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).

References

Submitted references

HLA class I antibodies trigger increased adherence of monocytes to endothelial cells by eliciting an increase in endothelial P-selectin and, depending on subclass, by engaging FcγRs.

Valenzuela NM, Mulder A, Reed EF
Journal of immunology (Baltimore, Md. : 1950) 2013 Jun 15;190(12):6635-50

Western blot

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Western blot testing of ThP-1 cell lysate with HLA-ABC antibody. Expected molecular weight of A/B/C: 40-41kDa.

Other assay

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

SDS-PAGE Analysis of Purified, BSA-Free HLA-ABC Antibody (clone 246-B8.E7). Confirmation of Integrity and Purity of the Antibody.