Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [3]
- Immunocytochemistry [1]
- Immunohistochemistry [10]
- Other assay [1]
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Validation data
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- Product number
- 17260-1-AP - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- HLA class I (HLA-B) Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Other
- Description
- Immunogen sequence: MRVTAPRTV LLLLSGALAL TETWAGSHSM RYFYTAMSRP GRGEPRFISV GYVDDTQFVR FDSDAASPRE EPRAPWIEQE GPEYWDRNTQ ICKTNTQTYR ESLRNLRGYY NQSEAGSHTL QRMYGCDVGP DGRLLRGHDQ YAYDGKDYIA LNEDLSSWTA ADTAAQITQR KWEAAREAEQ LRAYLEGLCV EWLRRYLENG KETLQRADPP KTHVTHHPIS DHEATLRCWA LGFYPAEITL TWQRDGEDQT QDTELVETRP AGDRTFQKWA AVVVPSGEEQ RYTCHVQHEG LPKPLTLRWE PSSQSTIPIV GIVAGLAVLA VVVIGAVVAT VMCRRKSSGG KGGSYSQAAS SDSAQGSDVS LTA (1-362 aa encoded by BC013187)
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 150 µL
- Concentration
- 0.29 mg/mL
- Storage
- -20°C
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Jurkat cells were subjected to SDS PAGE followed by western blot with 17260-1-AP (HLA class I (HLA-B) antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- MCF7 cells were subjected to SDS PAGE followed by western blot with 17260-1-AP (HLA class I (HLA-B) antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- HepG2 cells were subjected to SDS PAGE followed by western blot with 17260-1-AP (HLA class I (HLA-B) antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of HepG2 cells using 17260-1-AP (HLA class I (HLA-B) antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human spleen using 17260-1-AP (HLA class I (HLA-B) antibody) at dilution of 1:100 (under 10x lens).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human spleen using 17260-1-AP (HLA class I (HLA-B) antibody) at dilution of 1:100 (under 40x lens).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human heart using 17260-1-AP (HLA class I (HLA-B) antibody) at dilution of 1:100 (under 10x lens).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human heart using 17260-1-AP (HLA class I (HLA-B) antibody) at dilution of 1:100 (under 40x lens).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human kidney using 17260-1-AP (HLA class I (HLA-B) antibody) at dilution of 1:100 (under 10x lens).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human kidney using 17260-1-AP (HLA class I (HLA-B) antibody) at dilution of 1:100 (under 40x lens).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human lung using 17260-1-AP (HLA class I (HLA-B) antibody) at dilution of 1:100 (under 10x lens).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human lung using 17260-1-AP (HLA class I (HLA-B) antibody) at dilution of 1:100 (under 40x lens).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human ovary using 17260-1-AP (HLA class I (HLA-B) antibody) at dilution of 1:100 (under 10x lens).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human ovary using 17260-1-AP (HLA class I (HLA-B) antibody) at dilution of 1:100 (under 40x lens).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- IP result of anti-HLA class I (HLA-B) (IP:17260-1-AP, 4ug; Detection:17260-1-AP 1:2000) with HepG2 cells lysate 4000ug.