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- Antigen structure
- References [1]
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- Validations
- Western blot [1]
- Other assay [2]
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- Product number
- PA5-100822 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- HSPA4L Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- -20°C
Submitted references Polyamine metabolism links gut microbiota and testicular dysfunction.
Zhao Q, Huang JF, Cheng Y, Dai MY, Zhu WF, Yang XW, Gonzalez FJ, Li F
Microbiome 2021 Nov 11;9(1):224
Microbiome 2021 Nov 11;9(1):224
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of HSPA4L in COLO205 cell lysate (left lane: treated with the antigen-specific peptide). Samples were incubated with HSPA4L polyclonal antibody (Product # PA5-100822).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Fig. 4 Spermine ameliorated metabolites and influenced the offspring. a PCA score plot for testis metabolome (integration of ESI+ and ESI-) and metabolites improved by spermine (control vs TP: ** P < 0.01, and *** P < 0.001; TP vs SP+TP: * P < 0.05, ** P < 0.01, and *** P < 0.001; red represented the increase, while green represented the decrease, n = 6). b mRNA level of genes related to early and late spermatogenic events ( n = 6). c mRNA and protein level of HSP70s ( n = 6). d Total pups for each group and pups for each female mouse. e OPLS-DA score plot for plasma metabolome of the offspring (ESI+ and ESI- modes, n = 7). f Metabolites improved by spermine in plasma of the offspring ( n = 7). * P < 0.05, ** P < 0.01, and *** P < 0.001
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Fig. 5 HSP70 regulated by spermine protected against testicular toxicity induced by TP in mice and TM4 cells. a mRNA levels of HSP70s after treatment with spermine or eflornithine, a polyamine pathway inhibitor. b The function of HSP70 was indicated by cell viability, MDA, and LDH levels after treated with a HSP70 inhibitor VER155008. The concentration of spermine was 6.25 muM. c Spermine increased protein levels of HSP70s and eflornithine decreased protein levels of HSP70s. d VER155008 reduced the protective effect of spermine in mice. EF: eflornithine. The concentration of TP used in this experiment was 80 nM in TM4 cell. * P < 0.05, ** P < 0.01, and *** P < 0.001 ( n = 6)
