Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Immunocytochemistry [1]
- Flow cytometry [1]
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Validation data
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- Product number
- MAB4669 - Provider product page
- Provider
- R&D Systems
- Product name
- Human Integrin beta 7 Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. Detects human Integrin beta 7 in direct ELISAs and Western blots. In Western blots, no cross-reactivity with recombinant human Integrin beta 1, beta 2, beta 3, beta 4, beta 5, beta 6, beta 8, recombinant mouse Integrin beta 4, or beta 7 is observed.
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Unconjugated
- Antigen sequence
P26010
- Isotype
- IgG
- Antibody clone number
- 473207
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references Cellular Mechanisms of Etrolizumab Treatment in Inflammatory Bowel Disease.
Lichnog C, Klabunde S, Becker E, Fuh F, Tripal P, Atreya R, Klenske E, Erickson R, Chiu H, Reed C, Chung S, Neufert C, Atreya I, McBride J, Neurath MF, Zundler S
Frontiers in pharmacology 2019;10:39
Frontiers in pharmacology 2019;10:39
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Integrin beta 7 in Human PBMCs. Integrin beta 7 was detected in immersion fixed human peripheral blood mononuclear cells (PBMCs) using Mouse Anti-Human Integrin beta 7 Monoclonal Antibody (Catalog # MAB4669) at 15 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cell surfaces and cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of Integrin beta 7 in Human Lymphocytes by Flow Cytometry. Human peripheral blood lymphocytes were stained with Mouse Anti-Human Integrin beta 7 Monoclonal Antibody (Catalog # MAB4669, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG F(ab')2 Secondary Antibody (Catalog # F0102B).