Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
- Flow cytometry [1]
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Validation data
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- Product number
- AP53339PU-N - Provider product page
- Provider
- OriGene
- Product name
- PLCL2 (C-term) rabbit polyclonal antibody, Aff - Purified
- Antibody type
- Polyclonal
- Description
- PLCL2 (C-term) rabbit polyclonal antibody, Aff - Purified
- Host
- Rabbit
- Conjugate
- Unconjugated
- Epitope
- PLCL2
- Antibody clone number
- NULL
- Vial size
- 400 µl
- Concentration
- lot specific
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Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Western blot analysis of PLCL2 Antibody (C-term) ??in NCI-H460 cell line lysates (35ug/lane). This demonstrates the PLCL2 antibody detected the PLCL2 protein (arrow).
- Validation comment
- WB
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Confocal immunofluorescent analysis of PLCL2 Antibody (C-term) ??with NCI-H460 cell followed by Alexa Fluor 488-conjugated Goat anti-Rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red).DAPI was used to stain the cell nuclear (blue).
- Validation comment
- IF
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue reacted with PLCL2 Antibody (C-term) ?, which was?peroxidase conjugated to?the secondary antibody and?followed by?DAB staining. This data demonstrates the use of PLCL2 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.
- Validation comment
- IHC
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Flow cytometric analysis of NCI-H460 cells using PLCL2 Antibody (C-term) ? (right histogram) compared to a negative control cell (left histogram). FITC-conjugated donkey-anti-rabbit secondary antibodies were used for the analysis.
- Validation comment
- FC