MA5-35969

antibody from Invitrogen Antibodies

Targeting: ARID3B BDP, DRIL2

Western blot (Supportive data in Antibodypedia)

Immunoprecipitation (Data presented on provider website)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

MA5-35969

Provider

Invitrogen Antibodies

Product name

BDP Recombinant Rabbit Monoclonal Antibody (008)

Antibody type

Monoclonal

Antigen

Synthetic peptide

Reactivity

Human

Host

Rabbit

Isotype

IgG

Antibody clone number

008

Vial size

100 µL

Concentration

1 mg/mL

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot analysis of BDP using BDP Recombinant Monoclonal Antibody (Product # MA5-35969) at a dilution of 1:500. Lane A: 293T whole cell lysate, Lane B: K562 whole cell lysate, Lane C: HepG2 whole cell lysate, (Lysates/proteins at 30 µg per lane). The secondary antibody used was a Goat Anti-Rabbit IgG H&L (Dylight800) at 1:10,000 dilution. Developed using the Odyssey technique and performed under reducing conditions Predicted band size: 61 kDa. Observed band size: 68 kDa.

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunoprecipitation of BDP was performed on (Lane A) 0.5 mg K562 whole cell lysate (Lane B) 0.5 mg 293T whole cell lysate, using 2 µL of BDP Recombinant Monoclonal Antibody (Product # MA5-35969) diluted at 1:100 and 15 µL of 50% Protein G agarose. A Dylight 800-labeled antibody to rabbit IgG (H+L) was used as a secondary antibody at a 1:5000 dilution. Developed using the odssey technique and performed under reducing conditions. Predicted band size: 61 kDa. Observed band size: 61 kDa.