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Western blot
ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [4]
- Immunocytochemistry [1]
- Other assay [1]
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Validation data
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- Product number
- 10262-1-AP - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- PPP4C Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Other
- Description
- Immunogen sequence: MAEISDLDR QIEQLRRCEL IKESEVKALC AKAREILVEE SNVQRVDSPV TVCGDIHGQF YDLKELFRVG GDVPETNYLF MGDFVDRGFY SVETFLLLLA LKVRYPDRIT LIRGNHESRQ ITQVYGFYDE CLRKYGSVTV WRYCTEIFDY LSLSAIIDGK IFCVHGGLSP SIQTLDQIRT IDRKQEVPHD GPMCDLLWSD PEDTTGWGVS PRGAGYLFGS DVVAQFNAAN DIDMICRAHQ LVMEGYKWHF NETVLTVWSA PNYCYRCGNV AAILELDEHL QKDFIIFEAA PQETRGIPSK KPVADYFL (1-307 aa encoded by BC001416)
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 150 µL
- Concentration
- 0.27 mg/mL
- Storage
- -20°C
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- HEK-293 cells were subjected to SDS PAGE followed by western blot with 10262-1-AP (PPP4C antibody) at dilution of 1:0 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Mouse kidney tissue were subjected to SDS PAGE followed by western blot with 10262-1-AP (PPP4C Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- HEK-293 cells were subjected to SDS PAGE followed by western blot with 10262-1-AP (PPP4C antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Jurkat cells were subjected to SDS PAGE followed by western blot with 10262-1-AP (PPP4C Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of ( 10% Formaldehyde ) fixed HEK-293 cells using 10262-1-AP (PPP4C antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- IP result of anti-PPP4C (IP:10262-1-AP, 4ug; Detection:10262-1-AP 1:1000) with mouse kidney tissue lysate 4000ug.
