13812-1-AP

antibody from Invitrogen Antibodies

Targeting: NPHP4 KIAA0673, POC10, SLSN4

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

13812-1-AP

Provider

Invitrogen Antibodies

Product name

NPHP4 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Other

Description

Immunogen sequence: MNDWHRIFT QNVLVPPHPQ RARQPWKEST AFQCVLKWLD GPVIRQGVLE VLSEVECHLR VSFFDVTYRH FFGRTWKTTV KPTKRPPSRI VFNEPLYFHT SLNHPHIVAV VEVVAEGKKR DGSLQTLSCG FGILRIFSNQ PDSPISASQD KRLRLYHGTP RALLHPLLQD PAEQNRHMTL IENCSLQYTL KPHPALEPAF HLLPENLLVS GLQQIPGLLP AHGESGDALR KPRLQKPITG HLDDLFFTLY PSLEKFEEEL LELHVQDHFQ EGCGPLDGGA LEILERRLRV GVHNGLGFVQ RPQVVVLVPE MDVALTRSAS FSRKVVSSSK TSSGSQALVL RSRLRLPEMV G (1-350 aa encoded by BC040520)

Reactivity

Human, Mouse, Canine

Host

Rabbit

Isotype

IgG

Vial size

150 µL

Concentration

0.23 mg/mL

Storage

-20°C

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Mouse brain tissue were subjected to SDS PAGE followed by western blot with 13812-1-AP (NPHP4 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Y79 cells were subjected to SDS PAGE followed by western blot with 13812-1-AP (NPHP4 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

IF result from Dr. Corbit, Kevin. anti-NPHP4 (13812-1-AP) marks the ciliary axoneme, basal bodies, and the transition zone of Human hTERT-RPE1 cells.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of (4% PFA) fixed MDCK cells using 13812-1-AP (NPHP4 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L).

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human heart using 13812-1-AP (NPHP4 antibody) at dilution of 1:50 (under 10x lens).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human heart using 13812-1-AP (NPHP4 antibody) at dilution of 1:50 (under 40x lens).