TA501235

antibody from OriGene

Targeting: DDX39B BAT1, D6S81E, UAP56

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

TA501235

Provider

OriGene

Proper citation

OriGene Cat#TA501235, RRID:AB_11128787

Product name

BAT1 (UAP56) mouse monoclonal antibody, clone OTI2E4 (formerly 2E4)

Antibody type

Monoclonal

Description

BAT1 (UAP56) mouse monoclonal antibody, clone OTI2E4 (formerly 2E4)

Reactivity

Canine

Host

Mouse

Conjugate

Unconjugated

Epitope

DDX39B

Isotype

IgG

Antibody clone number

OTI2E4

Vial size

100 µl

Concentration

1 mg/ml

Western blot

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY BAT1 (RC201847, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-BAT1.

Validation comment

WB

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-BAT1 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).

Validation comment

WB

Immunocytochemistry

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Anti-BAT1 mouse monoclonal antibody (TA501235) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY BAT1(RC201847).

Validation comment

IF