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- References [33]
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- Product number
- 12544-1-AP - Provider product page
- Provider
- Proteintech Group
- Proper citation
- Proteintech Cat#12544-1-AP, RRID:AB_2195888
- Product name
- SQLE antibody
- Antibody type
- Polyclonal
- Description
- KD/KO validated SQLE antibody (Cat. #12544-1-AP) is a rabbit polyclonal antibody that shows reactivity with human, mouse, rat and has been validated for the following applications: IF, IHC, IP, WB, ELISA.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 20ul, 150ul
Submitted references Hibiscus syriacus L. cultivated in callus culture exerts cytotoxicity in colorectal cancer via Notch signaling-mediated cholesterol biosynthesis suppression.
A Novel Ferroptosis-Related Prognostic Signature Reveals Macrophage Infiltration and EMT Status in Bladder Cancer.
MYC Enhances Cholesterol Biosynthesis and Supports Cell Proliferation Through SQLE.
High SQLE Expression and Gene Amplification Correlates with Poor Prognosis in Head and Neck Squamous Cell Carcinoma.
p53 transcriptionally regulates SQLE to repress cholesterol synthesis and tumor growth.
HFD-induced TRAF6 upregulation promotes liver cholesterol accumulation and fatty liver development via EZH2-mediated miR-429/PPARα axis.
The antiviral enzyme viperin inhibits cholesterol biosynthesis.
The mammalian cholesterol synthesis enzyme squalene monooxygenase is proteasomally truncated to a constitutively active form.
Squalene epoxidase promotes colorectal cancer cell proliferation through accumulating calcitriol and activating CYP24A1-mediated MAPK signaling.
MiR-205-driven downregulation of cholesterol biosynthesis through SQLE-inhibition identifies therapeutic vulnerability in aggressive prostate cancer.
The cholesterol synthesis enzyme lanosterol 14α-demethylase is post-translationally regulated by the E3 ubiquitin ligase MARCH6.
Elevation of JAML Promotes Diabetic Kidney Disease by Modulating Podocyte Lipid Metabolism.
The MARCH6-SQLE Axis Controls Endothelial Cholesterol Homeostasis and Angiogenic Sprouting.
Valosin-containing protein mediates the ERAD of squalene monooxygenase and its cholesterol-responsive degron.
OSBPL2 deficiency upregulate SQLE expression increasing intracellular cholesterol and cholesteryl ester by AMPK/SP1 and SREBF2 signalling pathway.
Consulting prostate cancer cohort data uncovers transcriptional control: Regulation of the MARCH6 gene.
Impaired plasmalogen synthesis dysregulates liver X receptor-dependent transcription in cerebellum.
Squalene accumulation in cholesterol auxotrophic lymphomas prevents oxidative cell death.
FBXW7 regulates endothelial barrier function by suppression of the cholesterol synthesis pathway and prenylation of RhoB.
N-terminal acetylation and the N-end rule pathway control degradation of the lipid droplet protein PLIN2.
The ER membrane protein complex promotes biogenesis of sterol-related enzymes maintaining cholesterol homeostasis.
Ionizing Radiation induction of cholesterol biosynthesis in Lung tissue.
MARCH6 and TRC8 facilitate the quality control of cytosolic and tail-anchored proteins.
Haploid Mammalian Genetic Screen Identifies UBXD8 as a Key Determinant of HMGCR Degradation and Cholesterol Biosynthesis.
Genomic alterations underlie a pan-cancer metabolic shift associated with tumour hypoxia.
A MARCH6 and IDOL E3 Ubiquitin Ligase Circuit Uncouples Cholesterol Synthesis from Lipoprotein Uptake in Hepatocytes.
Dysregulation of Plasmalogen Homeostasis Impairs Cholesterol Biosynthesis.
The E3 ubiquitin ligase MARCH6 degrades squalene monooxygenase and affects 3-hydroxy-3-methyl-glutaryl coenzyme A reductase and the cholesterol synthesis pathway.
Reduction of the cholesterol sensor SCAP in the brains of mice causes impaired synaptic transmission and altered cognitive function.
Sterol homeostasis requires regulated degradation of squalene monooxygenase by the ubiquitin ligase Doa10/Teb4.
Cholesterol through the looking glass: ability of its enantiomer also to elicit homeostatic responses.
Cholesterol-dependent degradation of squalene monooxygenase, a control point in cholesterol synthesis beyond HMG-CoA reductase.
Diabetes and insulin in regulation of brain cholesterol metabolism.
Xu XY, Choi HS, Park SY, Kim JK, Seo KH, Kim H, Kim YJ
Phytomedicine : international journal of phytotherapy and phytopharmacology 2022 Jan;95:153870
Phytomedicine : international journal of phytotherapy and phytopharmacology 2022 Jan;95:153870
A Novel Ferroptosis-Related Prognostic Signature Reveals Macrophage Infiltration and EMT Status in Bladder Cancer.
Yan Y, Cai J, Huang Z, Cao X, Tang P, Wang Z, Zhang F, Xia S, Shen B
Frontiers in cell and developmental biology 2021;9:712230
Frontiers in cell and developmental biology 2021;9:712230
MYC Enhances Cholesterol Biosynthesis and Supports Cell Proliferation Through SQLE.
Yang F, Kou J, Liu Z, Li W, Du W
Frontiers in cell and developmental biology 2021;9:655889
Frontiers in cell and developmental biology 2021;9:655889
High SQLE Expression and Gene Amplification Correlates with Poor Prognosis in Head and Neck Squamous Cell Carcinoma.
Liu Y, Fang L, Liu W
Cancer management and research 2021;13:4709-4723
Cancer management and research 2021;13:4709-4723
p53 transcriptionally regulates SQLE to repress cholesterol synthesis and tumor growth.
Sun H, Li L, Li W, Yang F, Zhang Z, Liu Z, Du W
EMBO reports 2021 Oct 5;22(10):e52537
EMBO reports 2021 Oct 5;22(10):e52537
HFD-induced TRAF6 upregulation promotes liver cholesterol accumulation and fatty liver development via EZH2-mediated miR-429/PPARα axis.
Zhang Z, Wen H, Peng B, Weng J, Zeng F
Molecular therapy. Nucleic acids 2021 Jun 4;24:711-727
Molecular therapy. Nucleic acids 2021 Jun 4;24:711-727
The antiviral enzyme viperin inhibits cholesterol biosynthesis.
Grunkemeyer TJ, Ghosh S, Patel AM, Sajja K, Windak J, Basrur V, Kim Y, Nesvizhskii AI, Kennedy RT, Marsh ENG
The Journal of biological chemistry 2021 Jul;297(1):100824
The Journal of biological chemistry 2021 Jul;297(1):100824
The mammalian cholesterol synthesis enzyme squalene monooxygenase is proteasomally truncated to a constitutively active form.
Coates HW, Capell-Hattam IM, Brown AJ
The Journal of biological chemistry 2021 Jan-Jun;296:100731
The Journal of biological chemistry 2021 Jan-Jun;296:100731
Squalene epoxidase promotes colorectal cancer cell proliferation through accumulating calcitriol and activating CYP24A1-mediated MAPK signaling.
He L, Li H, Pan C, Hua Y, Peng J, Zhou Z, Zhao Y, Lin M
Cancer communications (London, England) 2021 Aug;41(8):726-746
Cancer communications (London, England) 2021 Aug;41(8):726-746
MiR-205-driven downregulation of cholesterol biosynthesis through SQLE-inhibition identifies therapeutic vulnerability in aggressive prostate cancer.
Kalogirou C, Linxweiler J, Schmucker P, Snaebjornsson MT, Schmitz W, Wach S, Krebs M, Hartmann E, Puhr M, Müller A, Spahn M, Seitz AK, Frank T, Marouf H, Büchel G, Eckstein M, Kübler H, Eilers M, Saar M, Junker K, Röhrig F, Kneitz B, Rosenfeldt MT, Schulze A
Nature communications 2021 Aug 20;12(1):5066
Nature communications 2021 Aug 20;12(1):5066
The cholesterol synthesis enzyme lanosterol 14α-demethylase is post-translationally regulated by the E3 ubiquitin ligase MARCH6.
Scott NA, Sharpe LJ, Capell-Hattam IM, Gullo SJ, Luu W, Brown AJ
The Biochemical journal 2020 Jan 31;477(2):541-555
The Biochemical journal 2020 Jan 31;477(2):541-555
Elevation of JAML Promotes Diabetic Kidney Disease by Modulating Podocyte Lipid Metabolism.
Fu Y, Sun Y, Wang M, Hou Y, Huang W, Zhou D, Wang Z, Yang S, Tang W, Zhen J, Li Y, Wang X, Liu M, Zhang Y, Wang B, Liu G, Yu X, Sun J, Zhang C, Yi F
Cell metabolism 2020 Dec 1;32(6):1052-1062.e8
Cell metabolism 2020 Dec 1;32(6):1052-1062.e8
The MARCH6-SQLE Axis Controls Endothelial Cholesterol Homeostasis and Angiogenic Sprouting.
Tan JME, van der Stoel MM, van den Berg M, van Loon NM, Moeton M, Scholl E, van der Wel NN, Kovačević I, Hordijk PL, Loregger A, Huveneers S, Zelcer N
Cell reports 2020 Aug 4;32(5):107944
Cell reports 2020 Aug 4;32(5):107944
Valosin-containing protein mediates the ERAD of squalene monooxygenase and its cholesterol-responsive degron.
Chua NK, Scott NA, Brown AJ
The Biochemical journal 2019 Sep 20;476(18):2545-2560
The Biochemical journal 2019 Sep 20;476(18):2545-2560
OSBPL2 deficiency upregulate SQLE expression increasing intracellular cholesterol and cholesteryl ester by AMPK/SP1 and SREBF2 signalling pathway.
Zhang C, Zhang H, Zhang M, Lin C, Wang H, Yao J, Wei Q, Lu Y, Chen Z, Xing G, Cao X
Experimental cell research 2019 Oct 15;383(2):111512
Experimental cell research 2019 Oct 15;383(2):111512
Consulting prostate cancer cohort data uncovers transcriptional control: Regulation of the MARCH6 gene.
Coates HW, Chua NK, Brown AJ
Biochimica et biophysica acta. Molecular and cell biology of lipids 2019 Nov;1864(11):1656-1668
Biochimica et biophysica acta. Molecular and cell biology of lipids 2019 Nov;1864(11):1656-1668
Impaired plasmalogen synthesis dysregulates liver X receptor-dependent transcription in cerebellum.
Honsho M, Dorninger F, Abe Y, Setoyama D, Ohgi R, Uchiumi T, Kang D, Berger J, Fujiki Y
Journal of biochemistry 2019 May 28;
Journal of biochemistry 2019 May 28;
Squalene accumulation in cholesterol auxotrophic lymphomas prevents oxidative cell death.
Garcia-Bermudez J, Baudrier L, Bayraktar EC, Shen Y, La K, Guarecuco R, Yucel B, Fiore D, Tavora B, Freinkman E, Chan SH, Lewis C, Min W, Inghirami G, Sabatini DM, Birsoy K
Nature 2019 Mar;567(7746):118-122
Nature 2019 Mar;567(7746):118-122
FBXW7 regulates endothelial barrier function by suppression of the cholesterol synthesis pathway and prenylation of RhoB.
Pronk MCA, Majolée J, Loregger A, van Bezu JSM, Zelcer N, Hordijk PL, Kovačević I
Molecular biology of the cell 2019 Mar 1;30(5):607-621
Molecular biology of the cell 2019 Mar 1;30(5):607-621
N-terminal acetylation and the N-end rule pathway control degradation of the lipid droplet protein PLIN2.
Nguyen KT, Lee CS, Mun SH, Truong NT, Park SK, Hwang CS
The Journal of biological chemistry 2019 Jan 4;294(1):379-388
The Journal of biological chemistry 2019 Jan 4;294(1):379-388
The ER membrane protein complex promotes biogenesis of sterol-related enzymes maintaining cholesterol homeostasis.
Volkmar N, Thezenas ML, Louie SM, Juszkiewicz S, Nomura DK, Hegde RS, Kessler BM, Christianson JC
Journal of cell science 2019 Jan 16;132(2)
Journal of cell science 2019 Jan 16;132(2)
Ionizing Radiation induction of cholesterol biosynthesis in Lung tissue.
Werner E, Alter A, Deng Q, Dammer EB, Wang Y, Yu DS, Duong DM, Seyfried NT, Doetsch PW
Scientific reports 2019 Aug 29;9(1):12546
Scientific reports 2019 Aug 29;9(1):12546
MARCH6 and TRC8 facilitate the quality control of cytosolic and tail-anchored proteins.
Stefanovic-Barrett S, Dickson AS, Burr SP, Williamson JC, Lobb IT, van den Boomen DJ, Lehner PJ, Nathan JA
EMBO reports 2018 May;19(5)
EMBO reports 2018 May;19(5)
Haploid Mammalian Genetic Screen Identifies UBXD8 as a Key Determinant of HMGCR Degradation and Cholesterol Biosynthesis.
Loregger A, Raaben M, Tan J, Scheij S, Moeton M, van den Berg M, Gelberg-Etel H, Stickel E, Roitelman J, Brummelkamp T, Zelcer N
Arteriosclerosis, thrombosis, and vascular biology 2017 Nov;37(11):2064-2074
Arteriosclerosis, thrombosis, and vascular biology 2017 Nov;37(11):2064-2074
Genomic alterations underlie a pan-cancer metabolic shift associated with tumour hypoxia.
Haider S, McIntyre A, van Stiphout RG, Winchester LM, Wigfield S, Harris AL, Buffa FM
Genome biology 2016 Jun 29;17(1):140
Genome biology 2016 Jun 29;17(1):140
A MARCH6 and IDOL E3 Ubiquitin Ligase Circuit Uncouples Cholesterol Synthesis from Lipoprotein Uptake in Hepatocytes.
Loregger A, Cook EC, Nelson JK, Moeton M, Sharpe LJ, Engberg S, Karimova M, Lambert G, Brown AJ, Zelcer N
Molecular and cellular biology 2016 Jan 15;36(2):285-94
Molecular and cellular biology 2016 Jan 15;36(2):285-94
Dysregulation of Plasmalogen Homeostasis Impairs Cholesterol Biosynthesis.
Honsho M, Abe Y, Fujiki Y
The Journal of biological chemistry 2015 Nov 27;290(48):28822-33
The Journal of biological chemistry 2015 Nov 27;290(48):28822-33
The E3 ubiquitin ligase MARCH6 degrades squalene monooxygenase and affects 3-hydroxy-3-methyl-glutaryl coenzyme A reductase and the cholesterol synthesis pathway.
Zelcer N, Sharpe LJ, Loregger A, Kristiana I, Cook EC, Phan L, Stevenson J, Brown AJ
Molecular and cellular biology 2014 Apr;34(7):1262-70
Molecular and cellular biology 2014 Apr;34(7):1262-70
Reduction of the cholesterol sensor SCAP in the brains of mice causes impaired synaptic transmission and altered cognitive function.
Suzuki R, Ferris HA, Chee MJ, Maratos-Flier E, Kahn CR
PLoS biology 2013;11(4):e1001532
PLoS biology 2013;11(4):e1001532
Sterol homeostasis requires regulated degradation of squalene monooxygenase by the ubiquitin ligase Doa10/Teb4.
Foresti O, Ruggiano A, Hannibal-Bach HK, Ejsing CS, Carvalho P
eLife 2013 Jul 23;2:e00953
eLife 2013 Jul 23;2:e00953
Cholesterol through the looking glass: ability of its enantiomer also to elicit homeostatic responses.
Kristiana I, Luu W, Stevenson J, Cartland S, Jessup W, Belani JD, Rychnovsky SD, Brown AJ
The Journal of biological chemistry 2012 Sep 28;287(40):33897-904
The Journal of biological chemistry 2012 Sep 28;287(40):33897-904
Cholesterol-dependent degradation of squalene monooxygenase, a control point in cholesterol synthesis beyond HMG-CoA reductase.
Gill S, Stevenson J, Kristiana I, Brown AJ
Cell metabolism 2011 Mar 2;13(3):260-73
Cell metabolism 2011 Mar 2;13(3):260-73
Diabetes and insulin in regulation of brain cholesterol metabolism.
Suzuki R, Lee K, Jing E, Biddinger SB, McDonald JG, Montine TJ, Craft S, Kahn CR
Cell metabolism 2010 Dec 1;12(6):567-79
Cell metabolism 2010 Dec 1;12(6):567-79
No comments: Submit comment
Supportive validation
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- A549 cells were subjected to SDS PAGE followed by western blot with 12544-1-AP(SQLE antibody) at dilution of 1:300
- Sample type
- cell line
Supportive validation
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- The SQLE antibody from Proteintech is a rabbit polyclonal antibody to a recombinant protein of human SQLE. This antibody recognizes human,mouse,rat antigen. The SQLE antibody has been validated for the following applications: ELISA, WB, IHC analysis.
