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Western blot
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [2]
- Immunoprecipitation [1]
- Immunohistochemistry [2]
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- Product number
- NBP2-04142 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Rabbit Polyclonal SRp55 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 ul
- Concentration
- 1.0 mg/ml
- Storage
- Store at 4C. Do not freeze.
Submitted references An activator of G protein-coupled receptor and MEK1/2-ERK1/2 signaling inhibits HIV-1 replication by altering viral RNA processing.
Wong RW, Balachandran A, Cheung PK, Cheng R, Pan Q, Stoilov P, Harrigan PR, Blencowe BJ, Branch DR, Cochrane A
PLoS pathogens 2020 Feb;16(2):e1008307
PLoS pathogens 2020 Feb;16(2):e1008307
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: SRp55 Antibody [NBP2-04142] - analysis of SRp55 in human lymphocytes using anti-SRp55 antibody. Each lane loaded with 14ug of whole cell lysate with 3 different human B-cell lines. Image from verified customer review.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: SRp55 Antibody [NBP2-04142] - CEM-T4 and HeLa whole cell lysates. Image from verified customer review.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunoprecipitation: SRp55 Antibody [NBP2-04142] - Samples: Whole cell lysate from HeLa (15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded), 293T (T; 50 ug), Jurkat (J; 50 ug) and mouse NIH3T3 (M; 50 ug) cells.Antibodies: Affinity purified rabbit anti-SRp55 antibody NBP2-04142 used for WB at 0.1 ug/ml (A) and 1 ug/ml (B) and used for IP at 6 ug/mg lysate. SRp55 was also immunoprecipitated by rabbit anti-SRp55 antibody, which recognizes an upstream epitope. Detection: Chemiluminescence with exposure times of 3 minutes (A) and 30 seconds (B).
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry: SRp55 Antibody [NBP2-04142] - Sample: FFPE section of human prostate carcinoma. Antibody: Affinity purified rabbit anti- SRp55 used at a dilution of 1:1,000 (1ug/ml). Detection: DAB. Counterstain: Hematoxylin (blue).
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry: SRp55 Antibody [NBP2-04142] - Sample: FFPE section of mouse teratoma. Antibody: Affinity purified rabbit anti- SRp55 used at a dilution of 1:1,000 (1ug/ml). Detection: DAB. Counterstain: Hematoxylin (blue).
